Electroacupuncture at Zusanli(ST36)Accelerates Intracerebral Hemorrhage-Induced Angiogenesis in Rats

【摘要】 <正>Objective:To investigate the effects of electro-acupuncture on intracerebral hemorrhage(ICH)induced angiogenesis and hypoxia-inducible factor-1α(HIF-1α) expression in rats.Methods:Adult male Sprague-Dawley rats were randomly divided into 4 groups of 24 rats each.ICH was induced in 3 groups by stereotactic injection of collagenase type VI into the right globus pallidus;of these,one group was not further treated,the second group underwent Zusanli(ST36)-acupuncture,and the third group underwent non-acupoint acupuncture. The fourth group underwent sham operations.Acupuncture was performed by stimulation with electrical needles at frequencies of 2-20 Hz for 30 min per day.Angiogenesis on days 3,7 and 14 was assessed by double immunolabeling,and expression of HIF-1αwas evaluated by immunohistochemistry,quantitative real time reverse transcription-polymerase chain reaction and Western blotting.Results:5-Bromo-2-deoxyuridine(BrdU)labeled nuclei in cerebral endothelial cells(ECs) resided around the hematoma and the labeling peaked from 7 to 14 days(P<0.01).HIF-1αpositive microvessels with a dilated outline were detected in perihematomal tissues after ICH,with the vessels extending into the clot from the surrounding area beginning on day 7.Following ICH, HIF-1αprotein levels increased(P<0.05),but HIF-1αmRNA levels did not change.Electro-acupuncture at the Zusanli(ST36) acupoint increased BrdU-labeled nuclei in cerebral ECs(P<0.05) and up-regulated the expression of HIF-1αprotein(P<0.05),but had little effect on the spatial distribution of HIF-1αor on HIF-1αmRNA levels.Conclusions:Electro-acupuncture treatment at the Zusanli(ST36) acupoint may accelerate ICH-induced angiogenesis by up-regulating HIF-1αprotein,and may enhance recovery following hemorrhagic cerebral injury.更多还原

【Abstract】 Objective:To investigate the effects of electro-acupuncture on intracerebral hemorrhage(ICH)induced angiogenesis and hypoxia-inducible factor-1α(HIF-1α) expression in rats.Methods:Adult male Sprague-Dawley rats were randomly divided into 4 groups of 24 rats each.ICH was induced in 3 groups by stereotactic injection of collagenase type VI into the right globus pallidus;of these,one group was not further treated,the second group underwent Zusanli(ST36)-acupuncture,and the third group underwent non-acupoint acupuncture. The fourth group underwent sham operations.Acupuncture was performed by stimulation with electrical needles at frequencies of 2-20 Hz for 30 min per day.Angiogenesis on days 3,7 and 14 was assessed by double immunolabeling,and expression of HIF-1αwas evaluated by immunohistochemistry,quantitative real time reverse transcription-polymerase chain reaction and Western blotting.Results:5-Bromo-2-deoxyuridine(BrdU)labeled nuclei in cerebral endothelial cells(ECs) resided around the hematoma and the labeling peaked from 7 to 14 days(P<0.01).HIF-1αpositive microvessels with a dilated outline were detected in perihematomal tissues after ICH,with the vessels extending into the clot from the surrounding area beginning on day 7.Following ICH, HIF-1αprotein levels increased(P<0.05),but HIF-1αmRNA levels did not change.Electro-acupuncture at the Zusanli(ST36) acupoint increased BrdU-labeled nuclei in cerebral ECs(P<0.05) and up-regulated the expression of HIF-1αprotein(P<0.05),but had little effect on the spatial distribution of HIF-1αor on HIF-1αmRNA levels.Conclusions:Electro-acupuncture treatment at the Zusanli(ST36) acupoint may accelerate ICH-induced angiogenesis by up-regulating HIF-1αprotein,and may enhance recovery following hemorrhagic cerebral injury.更多还原