【摘要】 目的观察雷公藤多苷含药血清对变应性接触性皮炎患者(allergic contact dermatitis,ACD)趋化因子γ干扰素诱导蛋白10(interferon-gamma-inducible protein-10,IP-10)、嗜酸性粒细胞趋化因子(Eosinophil chemoattractant chemokine,Eotaxin)mRNA表达的影响,进一步探讨雷公藤多苷治疗接触性皮炎的机制。方法逆转录聚合酶链式反应(Reverse Transcription Polymerase,RT-PCR)方法测定与不同浓度雷公藤多苷共同培养ACD患者外周血单一核细胞(PBMC)中IP-10、Eotaxin mRNA的表达。结果雷公藤多苷高、中、低3个浓度组体外培养的ACD患者PBMC中IP-10 mRNA表达明显低于对照组,差异均有统计学意义(P<0.01);雷公藤多苷含药血清3个浓度组之间比较,IP-10mRNA表达差异无统计学意义(P>0.05);雷公藤多苷含药血清3个浓度组之间及与对照组比较,体外培养的ACD患者PBMC中Eotaxin mRNA的表达差异均无统计学意义(P>0.05)。结论雷公藤多苷治疗ACD的机制之一可能是通过调节ACD患者PBMC中趋化因子IP-10、Eotaxin mRNA的水平,改变细胞因子的表达,促进Th1/Th2平衡来实现的。更多还原

【Abstract】 Objective To study the effect of Tripterygium Wilfordii Polyglycosidium(TWP) on allergic contact dermatitis(ACD) by detect the expression of chemokine interferon-gamma-inducible protein-10(IP-10) and Eosinophil chemoattractant chemokine(Eotaxin) messenger ribonucleic acid(mRNA).Methods Reverse Transcription Polymerase(RT-PCR) was used to detect the mRNA of IP-10 and Eotaxin in PBMC of ACD curtured with different concentration TWP.Results Compared with the control group,three different concentration groups(5.0,1.0,0.5 mg/L) of TWP all could remarkably suppress the expression of IP-10 mRNA in PBMC of ACD(P<0.01).While there was no significant difference among these three different concentration groups of TWP in there suppression on the expression of Eotaxin mRNA in PBMC of ACD(P>0.05).There was no significant different among the three different concentration groups of TWP on the expression of Eotaxin mRNA in PBMC of ACD(P>0.05).Conclusion TWP could regulate the expression of IP-10 and Eotaxin mRNA as well as cytokines expression ratio so as to modulate the Thl/ Th2 balance,which may be one of the mechanisms of TWP treatment on ACD.更多还原