【摘要】 [目的]筛选具有抗幽门螺杆菌(helicobacter pylori,HP)感染治疗价值的中药成分。[方法]摸索各中药成分的最低抑菌浓度,制备含有低于最低抑菌浓度中药提取液的HP的培养上清作用于Hela细胞,观察中药提取液对细胞空泡化的影响。[结果]各中药成分的最低抑菌浓度以黄连(0.39mg/ml)、厚朴(6.25mg/ml)、元胡(6.25mg/ml)最低,槟榔(25.00mg/ml)、黄柏(25.00mg/ml)、白芍(25.00mg/ml)等次之;药液作用后的细胞产生空泡的细胞的百分率以槟榔(8.0%)最低,厚朴(18.0%)次之。[结论]筛选到具有显著抑制细胞空泡化的中药成分为槟榔、厚朴,为临床治疗幽门螺杆菌感染提供了一种全新的思路。更多还原

【Abstract】 [Objective]To select the segment of Chinese medicine which has the potential value of antihelicobacter pylori(HP)infection.[Methods]Minimum inhibitory concentration(MIC)of segment of Chinese medicine was investigated.The extracting solution(lower than the MIC)of Chinese medicine,was added to culture medium with HP,and then the effect on Hela cells was observed.Cells vacuolation was also observed.[Results]The MIC of Coptis Chinensis,Cortex Magnoliae Officinalis and Corydalis Tuber was 0.39mg/ml,6.25mg/ml,6.25mg/ml respectively.The MIC of Betel Nut,Pellodendron and Radix Paeoniae Alba was 25mg/ml.Cell vacuolation rate induced by Betel Nut was the least(8.0%),that caused by cortex magnoliae officinalis was the second(18.0%).[Conclusion]We selected Chinese medicine Betel Nut and Cortex Magnoliae Officinalis,which could restrain the cell vacuolation.This would be a new way to treat HP infectional disease.更多还原