【摘要】 目的探讨血清胃蛋白酶原(PG)Ⅰ、Ⅱ和血清肿瘤相关物质(TAM)联合检测筛查胃癌的应用价值,为胃癌早期诊断提供新的理论和实验依据。方法选择80例受试者,其中胃癌患者40例,健康者40例,采用免疫比浊法测定血清PGⅠ、PGⅡ含量,并计算PGⅠ/PGⅡ比值;采用分光光度法测定血清TAM含量,分析联合检测血清PG和TAM对胃癌诊断的敏感性和特异性。结果与正常对照组相比,胃癌患者血清PGⅠ含量、PGⅠ/PGⅡ比值均显著降低(P<0.01),血清TAM含量明显升高(P<0.01),而血清PGⅡ含量无明显变化(P>0.05);单检胃癌患者血清胃蛋白酶原(PGⅠ及PGⅠ/PGⅡ)、TAM敏感性分别为75.00%、77.50%;特异性分别为72.50%、87.50%,两者联合检测在胃癌诊断中敏感性为92.50%,特异性为70.00%,联合检测的敏感性与单项检测的敏感性相比差异具有显著性(P<0.05)。结论联合检测血清胃蛋白酶原(PG)与血清肿瘤相关物质(TAM)筛查胃癌优于PG或TAM单筛,可明显提高胃癌早期诊断灵敏度。更多还原

【Abstract】 【Objective】To explore the clinical value of combined assay of serum pepsinogenⅠ(PGⅠ),pepsinogenⅡ(PGⅡ) and serum tumor association material(TAM) concentration for gastric cancer screening so as to provide new theoretical and experimental basis for the early diagnosis of gastric cancer. 【Methods】80 subjects which include 40 gastric cancers and 40 healthy controls were chosen to measure serum PGⅠ and PGⅡlevels by immunoturbidimetry and to calculate the ratio of the PGⅠand PGⅡ. Serum TAM levels were measured by spectrophotometry method. Then the sensitivity and specificity were analyzed when serum PG and serum TAM co-detection was used in the early diagnosis of gastric cancer. 【Results】Compared with normal control group,serum PGⅠand the ratio of the PGⅠand PGⅡ level in gastric cancer group significantly decreased(P <0.01),TAM level in gastric cancer group significantly increased(P <0.01),while the serum PGⅡ level in gastric cancer group had no notable variation(P >0.05). The sensitivity of single PG(PG Ⅰ and PG Ⅰ/ PG Ⅱ),TAM for diagnosis of gastric cancer were 75.00% and 77.50% respectively. Their specificitities were 72.50% and 87.50% respectively. The sensitivity and specificity of combined measurement of these two projects were 92.50% and 70.00% respectively,the sensitivity was shown to occur statistical difference(P <0.05). 【Conclusions】Combined detection of serum PG and TAM level is superior to single detection of PG or TAM for gastric cancer screening. This may improve sensitivity obviously when used in the early diagnosis of gastric cancer.更多还原