【摘要】 目的探讨急性坏死性胰腺炎(ANP)大鼠细胞因子水平与肠黏膜屏障损伤的相关性。方法制备ANP大鼠模型,检测假手术组和造模后6、12、24 h大鼠胰腺组织病理学、小肠黏膜TNF-α、IL-6,小肠组织occludin蛋白。组间数据比较采用单因素方差分析,TNF-a、IL-6与小肠组织occludin蛋白表达之间的相关性采用线性相关分析。结果模型组造模后,随造模时间的延长,小肠黏膜TNF-α、IL-6升高,小肠组织oc-cludin蛋白表达评分下降,各时点与同时点假手术组相比,差异均有显著性(P<0.05),TNF-a、IL-6与小肠组织occludin蛋白表达评分之间呈明显的负相关(P<0.01)。结论急性坏死性胰腺炎大鼠早期肠黏膜屏障损伤与肠黏膜TNF-α、IL-6水平升高有关。更多还原

【Abstract】 【Objective】 To discuss the correlation between the level of cytokine and intestinal mucosal barrier damage of acute necrotizing pancreatitis(ANP) in rats.【Methods】 This study establish acute necrotizing pancreatitis rat model and observe the histopathologic changes of pancreatic,the level of TNF-α,IL-6 in intestinal mucosa and expression of intestinal mucosa tight junction protein occludin in the two groups at 6,12,24 hours after establishment of model.The univariate analysis was used to compare the difference among groups.Linear correlation analysis was used to compare correlation between the level of TNF-α,IL-6 and intestinal mucosa tight junction protein occludin.【Results】 The level of TNF-α and IL-6 in intestinal mucosa were all significantly higher in model group at each time point after establishment of ANP model(P <0.05,vs sham group respectively).While expression of occludin in model group decreased gradually,and approached the minimum level at 24 hours as shown in IHC.It was lower than that in sham-operated group at each time point after establishment of ANP model(P <0.05).There was a negative relationship between cytokine(TNF-α,IL-6) and occludin obviously(P <0.01).【Conclusion】 Intestinal mucosa barrier damage is related to the increasing level of TNF-α,IL-6 in intestinal mucosa in the early stage of acute necrotizing pancreatitis in rats.更多还原