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6-BA和AgNO3对芥菜型油菜下胚轴芽再生的影响
Effects of 6-BA and AgNO3 on Shoot Regeneration of Brassica juncea(L.) Hypocotyls
【摘要】 以芥菜型油菜品种Brown mustard的下胚轴为外植体,接种于不同配比6-苄基腺嘌呤(6-BA)和硝酸银(AgNO3)组合的分化培养基中,研究6-BA和AgNO3对芥菜型油菜下胚轴芽再生的影响。结果表明分化培养基中含3 mg/L 6-BA+5 mg/L AgNO3时出芽率最高,出芽率达到58.33%,芽丛数平均为1.73。
【Abstract】 In order to study the effects of 6-BA and AgNO3 on shoot regeneration of Brassica juncea(L.) hypocotyls,we used the Brown mustard(Brassica juncea L.) hypocotyls as explant,which inoculated in differentiation medium of different concentration ratios of 6-benzyl adenine(6-BA) and silver nitrate(AgNO3) combination.The results revealed that the shoot regeneration rate was the highest when differentiation medium contained 3 mg/L 6-BA and 5 mg/L AgNO3,the bud rate reached 58.33% and the average bud number was 1.73.
【关键词】 芥菜型油菜;
下胚轴;
芽再生;
6-BA;
AgNO3;
【Key words】 Brassica juncea L; Hypocotyls; Shoot regeneration; 6-BA; AgNO3;
【Key words】 Brassica juncea L; Hypocotyls; Shoot regeneration; 6-BA; AgNO3;
【基金】 国家自然科学基金项目(31101176)
- 【文献出处】 作物研究 ,Crop Research , 编辑部邮箱 ,2013年02期
- 【分类号】S565.4
- 【被引频次】4
- 【下载频次】102