本研究对谷子株高等主要农艺性状进行了数量性状位点QTL分析。以表型差异较大的沈3/晋谷20F2作图群体为材料,观测其株高、穗长等性状,选用SSR作分子标记,利用完备区间作图法(BASTEN C J)进行QTL分析。结果显示,表型数据在作图群体中呈现连续分布,表现为多基因控制的数量性状,被整合的54个SSR标记构建10个连锁群,LOD阈值设置为2.0,检测到与株高相关的主效QTL2个,联合贡献率45.9637%,穗长主效QTL1个,贡献率14.9647%,与穗重、粒重相关的主效QTL为同一位点,贡献率分别为11.9601%和10.1879%。有6组QTL位点之间存在基因互作效应,大小范围为-0.4986~16.6407,对性状的贡献率在2.2716%~6.7478%之间。谷子表型控制复杂,相关QTL的检测受环境影响较大,不同连锁群QTL间互作明显。
【英文摘要】
Objective of the study is about to detect quantitative trait loci( QTLs) for such as plant height and panicle length of millet. F2population derived from crosses of the millet cultivars Shen3 × Jingu20 respectively was used for OTL analysis. Data of trait about plant height,panicle length and others was collected and recorded. By analysis,it said the traits recorded are all quantitative traits. Using SSR as molecular markers,54 markers were divided into 10 linkage group. The presence of two main-effect OTLs...
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