【摘要】 为了精确分离油松胚珠雌配子体,获得单一种类的细胞,进一步研究油松胚珠发育分子调控的时空特异性,本文利用激光显微切割技术(LCM)对2年生油松胚珠雌配子体进行了微切割;比较了冷丙酮(4℃)、卡诺固定液、FAA固定液固定及冻藏处理4种不同方法对冰冻切片的影响。结果表明,冻藏处理结合冰冻切片可以保证雌配子体的完整性,采用该方法获得了不同时期油松胚珠雌配子体,为后续油松胚珠雌配子体发育相关分子机制的研究提供了实验材料。更多还原

【Abstract】 In order to separate the ovules in female gametophyte accurately, acquire single cell type and further study the molecular mechanisms of the ovules in female gametophyte during development, laser capture microdissection (LCM) was used to isolate the ovules in female gametophyte of 2-year old Pinus tabulaeformis. And the effects of four methods, including cold acetone fixation (4 ℃), Carnoy’s fixation, FAA fixation and frozen storage, on frozen sections were compared. The results showed that frozen storage combined with frozen sections could ensure the intact morphology of female gametophyte, and obtain the female gametophytes from different developing stages. The method could provide experimental materials for investigating the molecular mechanisms of the development of ovules in female gametophyte of Pinus tabulaeformis.更多还原