【摘要】 目的探讨轻型颅脑损伤对大鼠脑源性神经营养因子(BDNF)基因表达的影响及意义。方法 120只成年SD大鼠根据性别不同分为雌性对照组、雌性实验组、雄性对照组和雄性实验组,每组30只。两实验组建立侧位液压冲击轻型颅脑损伤模型。Morris水迷宫试验测试大鼠学习记忆能力,RT-PCR检测海马中BDNF mRNA的表达。结果水迷宫定位航行试验第4天,同性别大鼠实验组平均逃逸潜伏期较对照组明显延长(P<0.05);空间探索试验同性别大鼠实验组寻找平台潜伏期较对照组明显延长(P<0.05),穿越平台次数较对照组减少(P<0.05);同性别大鼠实验组大脑海马中BDNF mRNA的表达显著低于对照组(P<0.01)。结论侧位液压冲击轻型颅脑损伤会降低模型鼠大脑海马中BDNF mRNA的表达,并可能因此导致大鼠学习记忆能力下降。更多还原

【Abstract】 Objective To explore the influence and significance of lateral fluid percussion-induced mild brain injury on the expression of brain-derived neurotrophic factor(BDNF) gene in rats.Methods According to gender,120 adult SD rats were divided into four groups: female control group,female experimental group,male control group and male experimental group,30 rats for every group.Two experimental groups were given lateral fluid percussion to create mild brain injury.The learning and memory function of rats were assayed by Morris water maze test,and the expression of BDNF mRNA in the hippocampus was detected by RT-PCR.Results The average escape latency was significantly longer in the experimental group than in the control group of same gender on the fourth day of place navigation test(P < 0.05).The latency in finding platform was significantly longer in the experimental group than in the control group of same gender,while the times for crossing the platform was significantly shorter in the experimental group than in the control group of same gender by spatial probe test(P < 0.05).The expression of BDNF mRNA was significantly lower in the experimental group than in the control group of same gender in the hippocampus of rats by RT-PCR test(P < 0.01).Conclusions The lateral fluid percussion-reduced mild brain injury can reduce expression of BDNF mRNA in the hippocampus of rats,and this may result in reduction of learning and memory ability in rats.更多还原