【摘要】 目的对一例A2B亚型患者进行血型血清学和分子生物学检测,观察采血400 ml前后主要血液指标的变化。方法用抗-A、抗-A1、抗-B、抗-H单抗血清检测患者红细胞ABH抗原;用A、B、O型标准红细胞与患者血清反应检测ABO血型抗体及不规则抗体;利用聚合酶链反应扩增患者ABO基因6、7外显子编码序列并进行测序分析;采用血液分析仪检测血液常规指标。结果患者红细胞上有A、B抗原,血清中存在抗-A1抗体,符合A2B亚型的血型血清学特征;患者ABO基因6、7外显子直接测序分析显示基因型为A208/B101,单倍体测定为A208、B101两个等位基因。采血前后及回输前后血常规主要指标无明显差别。结论发现1例A208/B101个体,表现出A2B血清学特性,血清中存在抗-A1抗体;符合要求的自体采血前后血液主要指标不会产生明显的变化。更多还原

【Abstract】 Objective To observe the changes of main blood index in a patient with A2B subgroup before and after blood sampling based on blood group serologic test and molecular biology test. Methods The blood group ABH antigen of erythrocyte from the patient was detected by anti-A,anti-A1,anti-B and anti-H antiserum. While the erythrocytes of A,B,O blood group reacted with serum of the patient to detect the ABO blood group antibody and irregular antibody. The coding region of exon6,7 of ABO gene was amplified by PCR for sequencing analysis,then blood analyzer was used to detect the conventional blood index. Results Both A and B antigen were found in erythrocyte of the patient,and anti-A1in serum of patient was in line with the blood group serologic characteristics of A2B subgroup. It can be assigned as A208 / B101 genotype by direct sequence analysis for exon 6,7 of ABO. A208 and B101 alleles were confirmed by ABO halpotype analysis. The main blood index had no obvious difference before and after blood sampling and transfusion. Conclusion An individual with A208 / B101 was found,showing the serological characteristic of A2B subgroup with anti-A1antibody in the serum. The main blood index had no obvious changes before and after the requested autologous blood collection.更多还原