【摘要】 目的建立食品中酸性橙Ⅱ、碱性橙2和碱性嫩黄测定的超高效液相色谱-串联质谱法。方法采用C18(2.1 mm×50 mm,1.7μm)色谱柱,以乙腈和0.1%甲酸的5 mmol/L乙酸铵水溶液为流动相,梯度洗脱,流速为0.3 ml/min,柱温40℃,碱性橙2、碱性嫩黄,ESI(+);酸性橙Ⅱ,ESI(-),在此色谱质谱条件下进行检测。结果该方法各组分检出限:酸性橙Ⅱ7.0μg/kg;碱性橙2 5.0μg/kg;碱性嫩黄5.0μg/kg,线性范围:酸性橙Ⅱ为7.0μg/kg~500.0μg/kg;碱性橙2和碱性嫩黄为5.0μg/kg~500.0μg/kg。加标回收率:酸性橙Ⅱ为93.5%~111.0%,;碱性橙2为93.5%~109.0%;碱性嫩黄为89.5%~108.0%。结论该方法操作简便,准确,快速,提高了样品检出灵敏度,能够同时检测3种非食用色素的含量。更多还原

【Abstract】 Objective To establish a UPLC-MS /MS method for determination of acid orangeⅡ,basic orange 2 and auramine O in foods. Methods Acid orangeⅡ,basic orange 2 and auramine O were separated on a C18 column using acetonitrile: ammonium acetate as the mobile phase at a flow rate of 0. 3 ml / min,with the column temperature at 40 C. Then basic orange 2and auramine O were detected with ESI( +),while acid orange Ⅱ was detected with ESI-. Results The detection limits of acid orangeⅡ,basic orange 2 and auramine O were 7. 0 μg / kg,2. 5 μg / kg and 5. 0 μg / kg respectively. The calibration curves were linear in the range of 7. 0 μg / kg ~ 500. 0 μg / kg for acid orange Ⅱ,5. 0 μg / kg ~ 500. 0 μg / kg for basic orange 2 and auramine O,while the recovery rates were 93. 5% ~ 111. 0%,93. 5% ~ 109. 0% and 89. 5% ~ 108. 0%. Conclusion The method is simple,precise,fast and suitable for determination of acid orangeⅡ,basic orange 2 and auramine O in foods.更多还原