【摘要】 目的监测和分析2011年-2012年度镇江市流行性感冒(流感)的流行动态和病原学特征,为流感的预防控制提供科学依据。方法对2011年-2012年镇江市流感监测哨点医院的流感样咽拭子标本先进行实时荧光定量PCR检测,核酸阳性标本接种狗肾传代(MDCK)细胞分离培养并鉴定病毒亚型。对血清标本用血凝抑制试验方法进行H1、H3、H1(2009)、B/Y和B/V亚型抗体检测,并对病原学结果和血清学结果进行统计分析。结果 2011年和2012年各收到流感样病例咽拭子标本1462份和1529份,其中核酸阳性数分别为96份(6.6%)和208份(13.6%)。对199份阳性标本进行了MDCK细胞病毒分离,分离出61株流感病毒(30.7%)。血清标本检测结果显示2011年-2012年镇江市人群中抗体阳性率均以H1亚型最高,分别为50%和55%。结论镇江市的流感流行高峰期为秋冬季和夏季,2011年以H1(2009)亚型和B型占主导优势,2012年以季节性H3亚型占主导优势。该市人群已获得一定的免疫力,但流感病毒仍比较活跃,需要继续加强监测,及时评价和预警流感流行情况。更多还原

【Abstract】 Objective To monitor and analyze the regular epidemic pattern and etiology features of influenza in Zhenjiang from 2011 to 2012,so as to provide scientific basis for prevention and control of influenza.Methods The throat swabs of influenza-like illness in the monitoring sentinel hospitals were collected and detected by real-time quantitative PCR first,then nucleic acid positive samples were isolated with Madin-Daby canine kidney( MDCK) cells to identify the subtypes.Serum samples were tested by hemagglutination inhibition assay on H1,H3,H1( 2009),B/Y and B/V subtype antibody.At the same time,the etiology and serology results of influenza was analyzed.Results A total of 1462 throat swabs were collected in 2011,and 96 samples were positive( 6.6%);while 1529 throat swabs were collected in 2012,and 208 samples were positive( 13.6%).One hundred and ninety-nine positive samples were isolated with Madin-Daby canine kidney( MDCK) cells,in which 61 strains were identified as influenza virus( 30.7%).Serum samples detection showed that the positive rate of H1 type was highest in antibody population in Zhenjiang from 2011 to 2012,50% and 55% respectively.Conclusion Summer,autumn and winter are the influenza fastigium in Zhenjiang.In 2011,H1( 2009) type and B type were prevalent,while H3 type was dominant in 2012.The crowd of this city has obtained certain immunity,but the influenza virus is still relatively active,need to continue to strengthen the monitoring,assessment and early warning of influenza epidemic situation in a timely manner.更多还原