【摘要】 目的:了解温州地区宫颈癌妇女人乳头瘤病毒(HPV)58 E6/E7基因变异情况,为HPV防治提供流行病学数据。方法:采用PCR方法扩增宫颈癌患者宫颈脱落细胞样本HPV58 E6/E7基因,DNA测序法获得基因序列,在GenBank进行经BLAST分析,寻找变异位点。结果:显示宫颈癌患者HPV58 E6变异率低于宫颈不典型增生、宫颈炎。在宫颈癌患者HPV58 E7变异率高于宫颈不典型增生、宫颈炎;E7最频繁变异C632T和G760A。年轻宫颈癌组与非年轻宫颈癌组E7 T20I/G63S变异率存在明显差异。结论:温州地区宫颈癌HPV58 E6较保守,而E7核苷酸变异明显,HPV58 E7 C632T/T744G/G760A变异可能与宫颈癌年轻化相关。更多还原

【Abstract】 Objective:To investigate the E6/E7 gene variation of human papillomavirus(HPV)58 in women with uterine cervical cancer,so as to provide epidemiological data for HPV prevention.Methods: Fifty cervical cancer patients were selected for HPV58 E6/E7 gene amplification by PCR,then the PCR products were carried out DNA sequencing to find the variable sites by BLAST analysis in the GenBank.Results: The rate of E6 nucleotide variation in cervical cancer patients was 10.00%,significantly lower than that of those with cervical dys plasia,cervicitis.The HPV58 E7 nucleotide variation rate in patients with cervical was 70.00%,significantly higher than that of those with cervical dysplasia,cervicitis;the most frequent sequence variation in the E7 was C632T,G760A;E7 nucleotide T20I/G63S variation rate was significantly different in the young cervical cancer group and non-young cervical cancer group.Conclusion: HPV58 E7 nucleotide variation rate was high,while the variation of E6 was low.In Wenzhou city,HPV E7 C632T/T744G/G760A variation may be closely associated with younger cervical cancer.更多还原