【摘要】 目的:研究15株多重耐药金黄色葡萄球菌耐药基因和肠毒素基因定位特征。方法:对15株多重耐药金黄色葡萄球菌质粒消除前后,进行药敏试验和肠毒素测定比较,从而对耐药基因和肠毒素基因进行定位。结果:15株多重耐药金黄色葡萄球菌耐药基因大多数位于质粒上,由质粒介导的耐药基因占70.6%,由染色体编码的耐药基因占29.4%。而对于肠毒素基因,发现SEA、SEC位于染色体上,SED位于质粒上,而SEB有位于染色体或质粒上。结论:耐药基因、肠毒素基因可以定位到染色体或质粒上,且质粒在决定耐药性中起主要作用。更多还原

【Abstract】 Objective:To investigate the resistant gene and enterotoxin gene of 15 strains of multiple-resistant staphylococcus aureus from food.Methods: The resistant genes and enterotoxin genes of staphylococcus aureus were detected before and after elimination of the plasmids and finding the location of those genes.Results: The resistant gene of 15 strains of staphylococcus aureus were mainly located in plasmids,the 70.6% of resistant gene was mediated by the plasmid and 29.4% of the resistant gene was mediated by the chromosome;the genetic locus of SEA,SEC were located in chromosome,and that of SED was located in plasmids,SEB was located in chromosome or plasmids.Conclusion: Antibiotics resistant gene and enterotoxin gene are located in both plasmids and chromosome.And plasmids play an important role in determining the resistant gene and enterotoxin gene.更多还原