【摘要】 目的:将Taqman荧光定量PCR技术与SYBRGreen染料RT-PCR技术比较后,应用于检测细菌NDM-1基因。方法:使用Taqman荧光定量PCR方法与SYBRGreen染料RT-PCR对已知特性的标准株中NDM-1基因检测,对比其扩增灵敏度。结果:Taqman荧光定量PCR技术灵敏度高于SYBRGreen染料RT-PCR法,所测标准株中NDM-1基因转录水平可高达7.5×104拷贝/μl,比SYBRGreen染料RT-PCR技术检测灵敏度高7.8倍。结论:Taqman荧光PCR技术对超级耐药菌NDM-1基因具有较高的敏感性和准确性,可以作为对超级细菌进行监控与防控的方法。更多还原

【Abstract】 Objective:In this study,TaqmanRT-PCR and SYBRGreenRT-PCR were applied for quantification of NDM-1 gene in bacteria.Methods: After detection of NDM-1 gene with Taqman RT-PCR and SYBRGreenRT-PCR,the sensititity was compared.Results: TaqmanRT-PCR was more sensitive and the resultant concentration of RNA level was up to 7.5×104 copies/μl.Compared with SYBR Green RT-PCR,it was up to 7.8-fold higher when determined by TaqMan RT-PCR.Conclusion: The detection of NDM-1 gene in bacteria with TaqmanRT-PCR was proved to be more sensitive and accurate,so Taqman RT-PCR could be suggested as a way to prevent and control super bacteria.更多还原