【摘要】 目的研究不同肝癌细胞对干扰素(IFN-γ)的敏感性是否存在差异。方法定量RT-PCR检测9种肝癌细胞系的IFN-γ受体表达,并通过比较IFN-γ处理前后HepG2、SNU182、SNU449和PLC4种细胞的周期变化和细胞凋亡情况,判定不同HCC细胞系对IFN-γ的敏感性差异。结果 HepG2、SNU449细胞IFN-γ受体IFNGR1表达低于正常肝组织(<0.5),Huh-7的IFNGR1和IFNGR2的表达均低于正常肝组织(<0.5)。IFN-γ能够诱导HepG2、SNU182和SNU449细胞凋亡,并能阻滞HepG2细胞的G1期向S期的细胞周期转换,SNU182细胞的G2/M期转换,但对PLC细胞生长抑制作用不明显。结论 IFN-γ可通过促进细胞凋亡和细胞周期阻滞抑制肝癌细胞生长,不同的肝癌细胞对IFN-γ敏感性存在差异,提示临床应用IFN-γ进行肝癌治疗时应考虑个体化差异。更多还原

【Abstract】 Objective To investigate the diversity of different hepatocellular carcinoma(HCC)cell lines in response to IFN-γtreatment in vitro.Methods Expression of receptors IFN-γin 9 kinds of HCC cell lines was detected using realtime(RT)-PCR.After IFN-γwas treated,the changes of cell cycle and proportion of apoptotic cell in HepG2、SNU182、 SNU449 and PLC cells were measured by flow cytometry.Results Low expression of IFNGR1 was observed in HepG2、 SNU449 and Huh-7 cell lines,low expression of IFNGR2 also exhibited in Huh-7 cell line(<0.5),when compared to normal liver tissues.IFN-γtreatment induced apoptosis of HepG2,SNU182 and SNU449 cell,cause cycle retardation in G1-S phase transition of HepG2 cell and G2/M arrest of SNU182 cell.No obvious inhibitory effects on PLC cell were observed.Conclusion IFN-γcould inhibit the proliferation of HCC by promoting cell apoptosis and cell cycle arrest.There are differences in HCC cells sensitivity to IFN-γ.In clinical practice,individual differences should be considered while HCC was treated with IFN-γ.更多还原