【摘要】 目的研究抑制性ODN对溃疡性结肠炎小鼠Th1/Th2免疫失衡的调节作用。方法首先利用我们已发表的研究[1]中的方法建立小鼠溃疡性结肠炎模型。建模成功后将结肠炎小鼠随机分为两组,分别给予腹腔内注射PBS、A151,记作PBS+UC组、A151+UC组,同时设一组健康小鼠对照并给予腹腔注射PBS,记作PBS+健康对照组。每组分别连续注射7天,末次注射后7天取小鼠血清并分离小鼠结肠组织制备匀浆。利用ELISA方法检测血清及结肠匀浆中IL-6、TNF-α、IL-10、IL-4水平。结果经检测发现抑制性ODN能明显下调IL-6和TNF-α的水平,而上调IL-10和IL-4水平。结论抑制性ODN对小鼠溃疡性结肠炎的抑制作用或许是通过调节Th1/Th2平衡来实现的。更多还原

【Abstract】 Objective To study if inhibitory ODN could induce Th1/Th2 immune balance induced by inhibitory ODN in mice with ulcerative colitis(UC).Methods Ulcerative colitis(UC)was induced by DSS in mice.The mice with UC were randomly divided into two groups.Mice with UC were injected with follows by i.p:PBS,A151,respectively. At 7days after last injection the mice were sacrificed.Serum and colons were taken out to undergo cytokines examination.The levels of cytokines were tested by enzyme linked immunosorbent assay(ELISA).Results A151 resulted in down-regulation of the level of IL-6 and TNF-α in serum and colonic tissue,and strikingly up-regulation of the level of IL-10 and IL-4.Conclusion The inhibitory ODN could therapy UC in mice by regulation of Th1/Th2 immune balance.更多还原