【摘要】 目的探讨CD4+CD25+CD127low/-调节性T细胞(Treg)在强直性脊柱炎(AS)活动期患者发病中的作用机制及其与C-反应蛋白(CRP)、血沉(ESR)的相关性。方法采用流式细胞术分别检测39例AS活动期患者和20例健康对照者外周血CD4+CD25+CD127low/-调节性T细胞占CD4+T细胞比例,同时分别检测两组C-反应蛋白(CRP)、血沉(ESR)的表达水平,并分析AS活动期患者CD4+CD25+CD127low/-调节性T细胞与CRP、ESR的相关性。结果 AS活动组外周血CD4+CD25+CD127low/-Treg与对照组比较明显降低,具有统计学意义(P<0.01)。AS活动组CRP、ESR与对照组比较均明显升高(P>0.05),具有统计学意义(P<0.01)。但AS活动期患者CD4+CD25+CD127low/-Treg表达率与CRP、ESR均未见明显相关性。结论 AS活动期患者CD4+CD25+CD127low/-Treg细胞数量的减少可能使免疫活性细胞逃避机体对它们的免疫调节,导致体内诱导免疫耐受机能不足,从而促进AS的发生发展。进行CD4+CD25+CD127low/-Treg的检测有助于评估AS活动期患者免疫状态,判断疾病进展,但其与CRP、ESR的相关性尚有待于进一步的研究。更多还原

【Abstract】 Objective To study CD4+CD25+CD127low/-regulator T cells on peripheral blood of antive ankylosing spondylitis(AS)pathogenesis,and analyze the relationship of CD4+CD25+CD127low/-Treg,C-reactive protein(CRP),erythrocyte sedimentation rate(ESR).Methods The sample consisted of 39patients with activated AS,the health control cluding 20patients.The expressions of CD4+CD25+CD127low/-Treg in CD4+T cell ratio were analyzed by flow cytometry,while detect the expression of CRP,ESR by markedlynd Westergren method,respectively.We analysis the correlation between Treg and CRP,ESR.Results CD4+CD25+CD127low/-Treg on peripheral blood of AS activity were lower than that in the healthy control group(6.19±1.51vs 7.50±1.62,P<0.01).CRP,ESR in AS activity were both markedly higher than that in control group(26.78±17.49vs 4.27±2.28;42.62±26.53vs 5.25±3.13,P<0.01).But the expression of CD4+CD25+CD127low/-Treg of AS activated patients had no correction with CRP and ESR.Conclusion The number of CD4+CD25+CD127low/-Treg reduction may cause immune cells to evade the body’s immune adjust,induce immune tolerance insufficiency,which contribute to the development of AS.Detection CD4+CD25+CD127low/-Treg help us to assess the state of immune and the progression of active AS.But the relationship of CD4+CD25+CD127low/-Treg and CRP,ESR still needed further research.更多还原