【摘要】 目的观察葛根素对心肌缺血再灌注损伤大鼠心肌酶的影响。方法以雄性Wistar大鼠为研究对象,将实验动物分为5组即假手术组、心肌缺血再灌注模型组(I/P)、葛根素2mg/Kg给药组(A组)、葛根素5mg/Kg给药组(B组)和葛根素10mg/Kg给药组(C组)。采用结扎大鼠冠状动脉左前降支(LAD)的方法,建立大鼠心肌缺血再灌注损伤模型;观察各组大鼠心肌梗死的面积及心肌酶的变化。结果与假手术组比较,I/P组大鼠的心肌梗死面积明显增大、心肌酶学各指标均发生显著变化,差异均有统计学意义(P<0.01);与I/P组比较,葛根素各剂量组均降低了心肌缺血再灌注损伤大鼠的心梗面积,减少了心肌细胞磷酸肌酸激酶(CK)、乳酸脱氢酶(LDH)释放、心肌组织黄嘌呤氧化酶(XO)活性及血清丙二醛(MDA)含量,提高了血清一氧化氮(NO)水平、血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及谷胱甘肽(GSH)含量,差异有统计学意义(P<0.01),并存在剂量反应关系。结论葛根素对心肌缺血再灌注损伤模型大鼠具有抗氧化应激作用,能够减轻心肌酶学的改变,从而减少心肌梗死面积,并存在剂量反应关系。更多还原

【Abstract】 Objective To observe the effect of puerarin on myocardial ischemiareperfu-sion injury in rat cardiac enzymes.Methods Male Wistar rats were randomly divided into sham group,myocardial ischemia-reperfusion model group,the puerarin 2 mg/Kg dose group(A),the the puerarin 5 mg/Kg dose group(B),the the puerarin 10mg/Kg dose group(C).Ligation of left anterior descending coronary artery(LAD),the establishmen-t of myocardial ischemia-reperfusion injury model;Rats myocardial infarct size and myocardial enzyme changes were observed.Results Myocardial infarct size compared with the sham group,I/P rats significantly increased serum creatine kinase targets were a significant change,the differences were statiscally significant(P<0.01);compared with the I/P group,Puerariahormone dose group were reduced myocardial ischemia-re-perfusion injury in rat infarct size,reduce of cardiomyocytes phosphate creatine kinase(CK),lactate dehydrogenase(LDH) release,myocardial tissue xanthine oxidase(XO)activity and serum malondialdehyde(MDA),increased serum nitric oxide(NO)levels,serum superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)activity and glutathionepeptide(GSH),the difference was statistically significant(P<0.01),and the existence of a dose-response relationship.Conclusion Puerarin on myocardial isch-emia-reperfusion injury model in rats with anti-oxidative stress effect can reduce the changes of serum crestine kinase,thereby reducing myocardial infarct size,and presence of a dose-response relationship.更多还原