【摘要】 为探讨联合用药对猪沙门菌耐药突变选择窗的影响,采用琼脂二倍稀释法测定环丙沙星、左氧沙星及其联合氨苄西林和卡那霉素对56株临床分离的猪源沙门菌的最低抑菌浓度(MIC)、耐药变异浓度(MPC)、耐药指数(SI),并进行统计学分析。结果显示,环丙沙星联合氨苄西林和卡那霉素后其耐药变异浓度(MPC)降低到2mg/L和1mg/L,SI分别下降了4倍和8倍;左氧沙星联合氨苄西林和卡那霉素MPC降低到1mg/L和0.5mg/L,SI分别下降了2倍和4倍;且两种药物与卡那霉素联合能完全关闭其突变选择窗(MSW)。本研究表明,环丙沙星、左氧沙星联合氨苄西林和卡那霉素能够缩小甚至关闭其单药的MSW,防止耐药的产生。更多还原

【Abstract】 To study the effect of antibiotics combination on the mutant selection window(MSW)of salmonella isolated from swine.The MIC of ciprofloxacin(CIP),levofloxacin(LVF),ampicillin(AMP) and kanamycin(KAN)and the mutant prevention concentration(MPC)both sole and combined were determined by the method of agar plates dilution method,and the value of SI(selection index:MPC/M IC) was acquired.SPSS statistics software was used to analyze the data.The results showed that after combined with ampicillin and kanamycin,the MPC of ciprofloxacin was reduced to 2mg/and 1mg/L,and the value of SI decreased by 4-and 8-fold respectively.The MPC of levofloxacin was reduced to 1mg/L and 0.5mg/L and the SI decreased by 2-and 4-fold.Compared with sole treatment,CIP and LVF combined with other two antibiotics can decrease MPC and SI of antibiotics alone and restrain the drug-resistant mutants.更多还原