【摘要】 为了研究T-2毒素对卵巢颗粒细胞(GC)分泌功能的影响及其作用机制。通过其染毒体外培养的大鼠GC,采用MTT法检测细胞相对活力、ELISA法检测收集培养液中孕酮(P)和雌二醇(E2)的含量、半定量RT-PCR检测激素分泌相关调控基因FSHR、StAR、P450scc和P450arom mRNA的表达、免疫细胞化学染色法检测细胞中特异的卵泡刺激素受体(FSHR)表达,结果显示,T-2毒素可显著降低体外培养的大鼠卵巢颗粒细胞活力,影响其激素的分泌,而转运蛋白StAR则可能是T-2毒素影响颗粒细胞激素分泌的关键位点之一。更多还原

【Abstract】 To study the toxic effects and explore the possible mechanism of T-2 toxin exposure to rat ovarian granulosa cell steroidogenesis in vitro,immature ovarian granulosa cells of wistar rat were primary cultured.After exposure to T-2 toxin 24h,cell viability was detected by MTT assay,content of progesterone(P) and estradiol(E2) in medium were detected by ELISA assay,FSHR,StAR,P450scc and P450arom mRNA expression were detected by semi-quantitative RT-PCR,and expression of follicle stimulating hormone receptor-specific receptor(FSHR) in granulosa cell was detected by immunocytochemical staining assay.The results showed that T-2 toxin could significantly decrease the viability of rat ovarian granulosa cells,and affected steroidogenesis,and that StAR maybe one of the key factors to affect the steroidogenesis of granulosa cells.更多还原