【摘要】 将60只健康试验大鼠随机分为3组:正常对照组(A组)、ET处理组(B组)、CA+ET处理组(C组),运用PCR-SSCP技术对肝脏mtDNA tRNACys-Tyr、COI基因片段一等基因进行了分析,同时运用分光光度计法对血清丙二醛的含量进行测定,观察ET致mtDNA的突变效应以及CA对该效应的保护作用。,结果发现,B组mtDNA tRNACys-Tyr基因片段在第5238与5242碱基处发生了插入1个碱基T的插入突变,COI一基因片段发生T5322G和T5331G处置换点突变,其他各组无基因突变发生。B组血清MDA含量均明显高于其他二组(P<0.05)、C组明显低于B组但高于A组(P<0.05)。结果表明,ET可导致mtDNA基因发生突变,而CA对ET致基因突变具有保护效应。更多还原

【Abstract】 To investigate the mutational effect of endotoxin(ET)-induced mtDNA and the protective effect of cation A(CA),sixty experimental rats were divided randomly into 3groups:normal control group(group A),ET treated group(group B),and CA+ET treated group(group C).The liver mtDNA was extracted for detecting the mutations of tRNACys-Tyr,COI fragment one gene by polymerase chain reaction-single stranded conformation polymorphism(PCR-SSCP)technique.The results revealed that a mutation was tRNACys-Tyr of group B,which was inserted T between 5238and 5242region,another mutation happened in T5322Gand T5331Gof the mtDNA COI one gene,no mutations of this gene was detected in group A and C.Serum MDA contents of group B were significantly higher than those of group A and C(P<0.05),those of group C were significantly lower than those of group B and higher than group A(P<0.05).It can be concluded that ET could cause the mutation of mtDNA gene,and CA has antagonistic and protective effect on the mutation of mtDNA gene caused by ET.更多还原