【摘要】 探讨反相微乳液法制备的β-纳米磷酸三钙/明胶/鹿茸多肽复合材料(简称纳米复合材料)对人成骨细胞功能的影响。利用反相微乳液法制备纳米复合材料,扫描电镜观察其形态,EDX与SELDI-TOF-MS分析对复合材料进行表征。采用MTT比色法、茜素红染色法检测纳米复合材料对体外培养的人成骨细胞hFOB1.19的增殖、矿化结节形成的影响;采用全自动生化分析仪检测hFOB细胞碱性磷酸酶活性(ALP)。结果表明该纳米复合材料可促进人成骨细胞增殖,提高ALP活性并增加矿化结节形成的数量,可促进人成骨细胞的增殖、分化成熟及矿化。更多还原

【Abstract】 The nano tricalcium phosphate/ gelatin /velvet antler polypeptide(VAP)composite materials(referred as nanocomposites) were prepared by water-in-oil(W/O) emulsion process.The effect of nanocomposites on human osteoblasts was studied in hFOB1.19 cell lines.Nanocomposites were prepared through emulsification-coacervation method in water-in-oil emulsions.The morphology was observed by scanning electron microscope.Characterization of composite spheres was examined by EDX and SELDI-TOF-MS.MTT assay and alizarin red staining were employed to detect the effects of nanocomposites on the proliferation and formation of mineralization nodules formation in hFOB cells,respectively.The alkaline phosphatase(ALP) activity was determined by automatic clinical chemistry analyzer.The results showed that nanocomposites could stimulate human osteoblasts cells proliferation increase ALP activity,and promote the formation of mineralization nodules.更多还原