【摘要】 运用荧光定量分析方法检测军牧1号白猪不同发育阶段睾丸组织miRNA-34b的表达量差异,结果:miRNA-34b在3月龄前的表达量都非常低,从5月龄开始表达量呈明显上升,7月龄达到峰值后开始明显下降。在猪的发育过程中,miRNA-34b的表达趋势曲线呈先增后降。结果表明miRNA-34b可能与猪雄性生殖发育相关。更多还原

【Abstract】 MicroRNAs(miRNAs) are short(-22 nucleotides) non-coding RNA molecules that play important roles by repressing translation or cleaving RNA transcripts.Some reports showed miRNA-34b is an important regulator in germ cell differentiation process of male humans and mice.We detected the different expressions of miR-34b of testicular tissue by real time quantitative PCR(RT-PCR) in Junmu No.1 white pig among the whole developmental stages.The results showed the expressions of miRNA-34b keep very low level before 3-month-old,rising dramatically from 5-month-old,reaching a peak at 7-month-old,and then descend apparently.Overview the expression trend of miRNA-34b in the whole sexual development processes,it runs a rule that first rising and then descending.The conclusion is anticipated that miR-34b keep a high possibility correlation with sexual development of male pig.更多还原