【摘要】 在应用实时荧光定量PCR法观察胰岛素(In)、胰高血糖素(GLN)、神经肽(NPY)对体外培养新生犊牛肝细胞硬脂酰CoA去饱和酶(Stearoyl CoA desaturase,SCD)mRNA丰度的影响。结果显示,随着培养液中In含量的升高,肝细胞中的SCD mRNA表达逐渐升高(P<0.05),呈现明显的剂量依赖促进效应;随着培养液中GLN含量的升高,肝细胞SCD mRNA丰度表达逐渐减弱,高血糖素处理组SCD mRNA表达均极显著低于对照组(P<0.01);而随着NPY质量浓度在0～1 000ng/L之间逐渐上升,肝细胞SCDmRNA的表达水平不断升高,各处理组显著高于对照组,除50ng/L处理组和500ng/L处理组之间差异不显著外,其他处理组之间差异显著(P<0.05)。结果表明,胰岛素和神经肽Y促进SCD mRNA表达,胰高血糖素抑制SCD mRNA表达。更多还原

【Abstract】 To order to investigate the regulation role of the insulin(In),glucagon(GLN),and neuropeptide(NPY) in hepatocyte glycometabolism and lipometabolism of dairy cows,effects of In,GLN and NPY on expression of stearoyl-CoA desaturase(SCD) mRNA in neonatal calf hepatocytes cultured in vitro were observed by real-time quantitative PCR.The production of SCD mRNA increased along with increase of levels of In,and there is a significant difference amongall group(P<0.05);when increased gradually glucagon in the culture media,the production of SCD mRNA decreased,and there is extremely significant difference among all group(P<0.01);the SCD mRNA express of hepatocytes is increased by NPY ranged from 0 to 1 000 ng/L(P<0.05),and there is significant difference among other group except treated with 50 ng/L and 500 ng/L(P<0.05).These results demonstrated that In and NPY in the medium can directly increase the expression of SCD mRNA,but glucagon can depress it,so the three factors can regulate the expression of SCD mRNA in the neonatal calf hepatocytes in vitro.更多还原