【摘要】 为了拓宽秸秆的生物降解应用研究,深入探讨白腐菌的木质素降解途径与机制,本研究采用平板变色法对12个糙皮侧耳菌株进行初步筛选,再通过棉秸秆木质素降解实验对初选菌株进行复筛。结果表明,初步筛选试验中选出了漆酶和过氧化物酶分泌能力都较强的8个菌株,即皖平1号、P17、早、黑平A、苏平1号、苏平3号、新平400和天达300。木质素降解试验复筛出高效降解木质素的糙皮侧耳菌株为苏平1号、皖平1号、苏平3号、黑平A,其中以苏平1号对棉秸秆木质素的降解能力最强,其木质素降解率为14.82%。8株菌株在棉秸秆与棉籽壳栽培中的生物转化率差异不显著,筛选出的高效降解秸秆木质素的菌株较其他菌株在棉秸秆栽培中产量、生物转化率均较高,其中苏平1号的产量、生物转化率最高,黑平A次之。从12个糙皮侧耳菌株中筛选出了高效降解棉秸秆木质素的菌株4株,即苏平1号、皖平1号、苏平3号、黑平A,其在秸秆栽培应用中产量、生物转化率也较高,尤以苏平1号、黑平A为较优,有重要的应用价值。更多还原

【Abstract】 To study out the white rot fungi lignin degradation pathways and mechanisms and broad the application research of straw biological degradation,chromogenic tests were used for preliminary screenings of 12 Pleurotus ostreatus strains and lignin degradation experiments as further screening methods. The results showed that in initial screening tests the 8 strains which had strong capabilities of laccase and peroxidase secretion were as followed: strains of Wanping No1,P17,Zao,Heiping A,Suping No1,Suping No3,Xinping 400 and Tianda 300. Lignin degradation test screened out 4 strains which efficiently decomposed lignin of cotton straw,such as strains of Suping No1,Wanping No1,Suping No3,Heiping A. And Suping No1 had the strongest lignin degradation ability of cotton straw,the degradation rate was 14. 82%. In the mushroom cultivation experiment of those 8strains,there were no significant differences in biological efficiency of fruiting body between cotton straw cultivation and cottonseed cultivation. But the strains which were screened out were with more fruit body yield and higher conversion rate than other strains. Suping No1 still had the most fruit body yield and highest conversion rate before Heiping A. In 12 Pleurotus ostreatus strains,4 strains were screened out as efficiently decomposing lignin of cotton straw strains,such as strains of Suping No1,Wanping No1,Suping No3,Heiping A. In straw cultivation application,the fruiting body yields and conversion rates of the 4 strains were higher than others,especially for Suping No1 and Heiping A. This results had great application value.更多还原