【摘要】 对采集于云南省弥勒县的青头菌采用组织分离法于PDA、改良MMN及实验室改良培养基平板上进行分离比较。结果显示,改良MMN培养基对菌柄分离效果最佳,分离物经ITS序列测定,系统发育分析为青头菌。更多还原

【Abstract】 The fruiting body of Russula virescens collected from Mi’le,Yunnan Province,was used to obtain pure culture.The fresh tissues were placed on PDA,the improved MMN and an improved medium,respectively,to compare the successful rate of isolation.We found that the improved MMN was the best medium for isolation from the stipe of mushroom.The isolate was identified as Russula virescens analyzed by phylogenetic relationship of internal transcribed spacer(ITS) gene sequences.更多还原