【摘要】 目的探讨原花青素对大肠癌HT-29细胞增殖及Pim-1、Bcl-2、CyclinD1基因的影响。方法取对数生长期HT-29细胞,分别加入100 mg/L、200 mg/L、400 mg/L、800 mg/L原花青素培养,采用四甲基偶氮唑蓝(MTT)法检测细胞增殖;采用荧光定量PCR检测Pim-1、Bcl-2、CyclinD1 mRNA表达。结果 24 h时,各浓度组原花青素对HT-29细胞增殖抑制率分别为(4.28±0.34)%、(10.05±0.61)%、(16.67±0.63)%、(28.16±0.91)%;48 h时,各浓度组原花青素对HT-29细胞增殖抑制率分别为(11.47±1.53)%、(17.65±1.21)%、(30.82±1.99)%、(44.48±2.18)%,对Pim-1、Bcl-2、CyclinD1mRNA表达有抑制作用(P<0.05),且作用呈浓度依赖性。结论原花青素体外可呈浓度依赖性抑制HT-29细胞增殖,其机制可能与抑制Pim-1、Bcl-2、CyclinD1表达有关。更多还原

【Abstract】 Objective To investigate the effects of procyanidin(PA) on proliferation in human colon carcinoma cell line HT-29.Methods Human colon carcinoma cell line HT-29 of logarithm veget al period were cultured with different density of PA(100,200,400,800 mg/L) for 48 hours,the suppression rate of cell proliferation was assayed by MTT method.The expression of Pim-1、Bcl-2、CyclinD1 was measured by fluorescent quantitative PCR respectively.Results The suppression rate of cell proliferation was(4.28±0.34)%、(10.05±0.61)%、(16.67±0.63)%、(28.16±0.91)% separately at 24 hours and was(11.17±2.36)%、(17.88±1.77)%、(31.65±7.03)%、(44.18.65±5.21)% differently at 48 hours,and the Pim-1、Bcl-2、CyclinD1 expression was reduced significantly after 48 h(P< 0.05).What’s more,the effects were in a dose-dependent manner.Conclusion PA could concentration-dependently inhibit the growth of HT-29 cells,and the mechanism could possibly contribute to reduce Pim-1、Bcl-2、CyclinD1 expression levels.更多还原