【摘要】 目的了解2006—2011年西安市污染食品及食物中毒中金黄色葡萄球菌(S.aureus)分离株肠毒素(SEs)、杀白细胞素(PVL)、表皮剥落毒素(ETs)、毒性休克综合征毒素-1(TSST-1)等毒素基因的分布状况,并比较两种分离株在基因分布及分型上的差异。方法采用多重PCR法检测61株S.aureus(包括40株食品分离株和21株食物中毒分离株)sea、seb、sec、sed、pvl、eta、etb、tsst-1基因,其中sea、seb、sec、sed基因引物加入同一反应体系,剩余4对基因引物加入另一反应体系。结果 40株食品分离株中17株检出毒素基因(42.50%);21株食物中毒分离株中18株检出毒素基因(85.71%),食物中毒分离株毒素基因的检出率明显高于食品分离株(P<0.01)。食品分离株中主要流行的毒素基因为sea(25%)、eta(12.5%),未检测到携带etb、tsst-1基因的菌株;同时得到8种毒素基因型,主要流行的基因型为sea(10.00%)、sea+eta(7.50%)。食物中毒分离株中主要流行的毒素基因为sea(76.19%)、sec(28.57%),未检测到携带pvl基因的菌株;同时得到6种毒素基因型,主要流行的基因型为sea(42.86%)、sea+sec+tsst-1(14.29%)。结论 S.aureus食品分离株和食物中毒分离株在毒素基因的分布及分型上存在较大差异。更多还原

【Abstract】 Objective To obtain an overview of epidemic characteristics of Staphylococcal enterotoxins(SEs),Panton-Valentine leukociclin(PVL),exfoliative toxins(ETs),and toxic shock syndrome toxin1(TSST-1) genes of Staphylococcus aureus(S.aureus) isolated from food and food poisoning samples in Xi’an from 2006 to 2011,and to compare the difference of gene distribution and genotyping between the two kinds of isolates.Methods The genes of sea,seb,sec,sed,eta,etb,tsst-1 and pvl were detected by multiplex PCR.The multiplex PCR assay combined the primers of sea,seb,sec and sed in one reaction and the other four primers in another.Results Out of 40 S.aureus strains isolated from food,17 strains were detected toxin genes(42.50%).In 21 strains isolated from food poisoning,18 strains were detected toxin genes(85.71%).The detection rate of toxin genes in food poisoning isolates was much higher than that in food isolates(P<0.01).Sea(25%) and eta(12.5%) were the most common genes and sea(10.00%) and sea+eta(7.50%) were the main toxin genotypes in food isolates,and etb,tsst-1 genes were not detected.Sea(76.19%) and sec(28.57%) were the most common genes and sea(42.86%) and sea+sec+tsst-1(14.29%) were the main toxin genomic types in food poisoning isolates,and PVL gene were not detected.Conclusion There were significant differences in toxin gene distribution and genotyping between S.aureus isolates from food and isolates from food poisoning.更多还原