【摘要】 目的了解宁波地区食品中携带或污染的致病菌,为控制食源性疾病提供依据。方法致病菌检测采用直接分离与增菌分离相结合的方法;细菌鉴定采用生化筛检和API等方法;血清分型采用诊断血清凝集法;药敏试验采用K-B法;采用PCR检测耐药基因。结果从6 812份食品标本中检出致病菌7类12种,共2 331株,检出率为34.22%,以副溶血性弧菌检出率最高,与其他病原菌检出率比较差异有统计学意义(P<0.005)。主要流行株副溶血性弧菌分型发现10个血清群,O∶6群和O∶5群为优势群。检出的致病菌对大多数抗生素敏感,其中3株气单胞菌为带aacc耐药基因的多重耐药菌。结论宁波地区食品中致病菌构成复杂,食品污染是引起食源性疾病的主要原因,其中副溶血性弧菌是最主要的流行菌株;检出的致病菌对多种抗生素敏感,存在aacc耐药菌应引起关注,控制的关键是采取合理用药,加强对耐药菌株的监测,以减少耐药菌的传播和扩散。更多还原

【Abstract】 Objective objective To understand the presence,contamination and cross contamination of foodborne bacterial pathogens in Ningbo city,provide basis for foodborne disease control,and trace the source of foodborne disease.Methods Strains were detected directly or after enrichment with biochemistry and API method,and subtyped with serum agglutination method.Antibiotic resistance and relative genes were detected with K-B method and PCR method respectively.Results 2 331(7 species and 12 types)strains were detected from 6 812 food samples and the detection rate is 34.22%(2 331/6 812).The prevalent pathogens were Vibrio parahaemolyticus,and the detection rate was significantly different from the other types(P<0.005).Vibrio parahaemolyticus could be classified into 10 sero-groups,and O6 and O5 were proved as the prevalent sero-groups.Most of the pathogens were sensitive to antibiotics.Three strains of Aeromonas were found multi-resistant with aacc resistance gene.Conclusion Various distribution was proved in foodborne bacteria in Ningbo.Contamination of foodborne pathogens was a major factor of foodborne diseases.Vibrio parahaemolyticus was the prevalent pathogenic bacteria.Most of the pathogens were sensitive to antibiotics.Bacteria with aacc resistance gene were found,which should raise concerns to control the spread of the resistant strains through rational administration of antibiotics and resistance surveillance.更多还原