【摘要】 目的研究蜂胶黄酮及其功效成分对脂质代谢紊乱的调节作用及机制。方法建立高脂血症大鼠模型,研究蜂胶黄酮对大鼠血脂水平及脂质代谢关键酶活性的影响;同时,在体外观察蜂胶黄酮功效成分对胰岛素抵抗的改善作用。结果蜂胶黄酮可显著降低高脂血症大鼠的血清甘油三酯(TG)和总胆固醇(TC)水平(P<0.05);在体外试验中,蜂胶黄酮可上调肝酯酶(HL)和7α-羟化酶(7CYP7A1)的活性水平(P<0.05);在胰岛素抵抗的L6肌管细胞中,蜂胶黄酮功效成分7-甲基白杨素、槲皮素-3-O-新橙皮糖苷和咖啡酸可明显提高细胞对葡萄糖的消耗(P<0.05);7-甲基白杨素0.4μg/ml以及槲皮素-3-O-新橙皮糖苷10μg/ml能显著降低L6肌管细胞对游离脂肪酸(FFA)的摄取。结论蜂胶黄酮可有效改善脂质代谢紊乱,其作用机制可能是增强脂质代谢关键酶活性以及改善胰岛素抵抗状况。更多还原

【Abstract】 Objective To study the effect and mechanism of propolis flavonoids and its effective component on the regulation of lipid metabolism.Methods The effect of propolis flavonoids on serum lipids(TG,TC and HDL-C) and the key enzyme in lipid metabolism(HL and 7CYP7A1)were tested in hyperlipidemia rat model and primary cell line(hepatocyte and adipocyte) respectively;and the effect of the active component from propolis flavonoids on insulin resistance was observed in vitro as well.Results The levels of TG and TC in propolis flavonoids group were significantly decreased compared with the group of hyperlipidemia model(P<0.05).The levels of HL and 7CYP7A1 were significantly increased compared with the group of hyperlipidemia model(P<0.05) in vitro.The effective component of propolis flavonoids,including 7-methylchrysin,quercetin-3-O-neohesperidin and caffeic acid,increased the glucose consumption in L6 myotubes(P<0.05).Moreover,7-methylchrysin in the dose of 0.4 μg/ml and quercetin-3-O-neohesperidin in the dose of 10 μg/ml could significantly reduce free fatty acids absorption in L6 myotubes.Conclusion The mechanism of propolis flavonoids on the regulation of lipid disorders might be depend on the regulation of the key enzyme in lipid metabolism and the insulin resistance.更多还原