【摘要】 目的观察不同浓度的锶矿泉水对人脐静脉血管内皮细胞(HUVEC)增殖及分泌的影响,研究其对血管内皮细胞功能的作用。方法 HUVEC细胞分为2、4、6、8 mg/L锶矿泉水(SMW)组和双蒸水(DDW)组,以4×104个/ml的浓度接种于培养板中,18 h后,换成条件培养基培养至96 h。分别于24、48、72和96 h时,以细胞计数试剂盒-8(CCK-8)法观察细胞的增殖情况。培养72 h的细胞分别用苏木素染色后,观察细胞生长状态,以及用BCA蛋白化验试剂盒测蛋白含量,酶联免疫吸附试验(ELISA)检测培养基中血管内皮生长因子(VEGF)、一氧化氮(NO)和内皮素(ET)的含量。结果培养72 h后,2和4 mg/L SMW组细胞增殖速度快于DDW组(P<0.05);8 mg/LSMW抑制HUVEC增殖(P<0.05);培养至72 h时,各浓度的SMW组细胞分泌VEGF的量较DDW组均降低(P<0.05),其组间差异无统计学意义(P>0.05);2和4 mg/L SMW组分泌ET和NO减少(P<0.05),而6和8 mg/LSMW组分泌增多(P<0.05),但同一浓度下,ET和NO分泌变化呈正相关。4 mg/L SMW组NO/ET值增大(P<0.05),其余各组间差异无统计学意义(P>0.05)。结论一定浓度的锶矿泉水可促进血管内皮细胞增殖,影响内皮细胞ET和NO分泌,降低血管紧张性。VEGF并不直接参与锶矿泉水促进血管内皮细胞的增殖。更多还原

【Abstract】 Objective To observe the effects of strontium mineral water(SMW) on proliferation and secretion of human umbilical vein endothelial cells(HUVEC) at different strontium concentration.Methods HUVEC were cultured in vitro.After culturing in a normal Dulbecco’s Modified Eagle’s Medium(DMEM) for 18 hours,the cells were transferred to the conditioned medium with 2,4,6,8 mg/L strontium mineral waters and double distilled water(DDW) treatment.The proliferation of cells was observed by Cell Counting Kit-8(CCK-8) assay at 24th,48th,72nd and 96th hours.Cell growth was observed by hematoxylin staining at 72nd hours.Meanwhile,cell protein was measured by Enhanced BCA Protein Assay kit and levels of extracellular vein endothelial growth factor(VEGF),nitric oxide(NO) and endothelin(ET) were tested by enzyme-linked immuno sorbent assay(Elisa).Results The proliferation activity of cells in 2 and 4 mg/L SMW groups were significantly increased(P<0.05),while significantly decreased in 8 mg/L SMW group(P<0.05) after 72 hours.Total of extracellular VEGF in SMW groups were lower than DDW group(P<0.05),and there was no significant difference between SMW groups(P>0.05) after 72 hours.NO and ET of cells in 2 and 4 mg/L groups were hyposecretion,while 6 and 8 mg/L groups were on the contrary.NO/ET ratio of 4 mg/L SMW group was higher than the others(P<0.05),and the difference of NO/ET ratio between the other groups was not significant(P>0.05).Conclusion A certain concentration of strontium in mineral water is good for proliferation of HUVEC.It could affect HUVEC secretion function of ET and NO,and decrease vascular tone.VEGF was not the direct factor which enhanced the proliferation activity of HUVEC culturing in strontium mineral water.更多还原