【摘要】 目的:观察复方降脂胶囊(Fufang Jiangzhi capsule,FFJZ)对颈动脉内膜损伤后高脂诱导动脉粥样硬化(AS)大鼠血脂及炎症因子的影响。方法:SD大鼠50只,雌雄各半,右侧颈总动脉损伤、予高脂饮食、维生素D3肌肉注射复制动脉粥样硬化模型(AS)。按体重随机分为模型组(生理盐水)、洛伐他汀组(7.48 mg·kg-1)、FFJZ高、中、低(按生药量计为53.34,26.67,13.33 g·kg-1)剂量组;另取10只正常大鼠作为空白组(生理盐水),予普通饲料饲养。每天灌胃1次,给药容积为10 mL·kg-1。给药12周后取血及右侧颈总动脉标本,检测血液中血脂及白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)的含量,并作病理组织切片,光镜下观察动脉壁变化情况。结果:造模12周后,模型组动物血液中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C),IL-6,TNF-α的含量升高,高密度脂蛋白胆固醇(HDL-C),IL-10的含量降低,与空白组比较具有显著性差异(P<0.05或P<0.01)。与模型组比较,经FFJZ干预治疗可降低实验动物血液中TG,TC,LDL-C,IL-6,TNF-α的含量,升高血液中HDL-C,IL-10,的含量,并减少动脉管壁斑块的生成,降低内膜中泡沫细胞与脂质的沉积,减少AS斑块中巨噬细胞数量,与模型组比较具有显著性差异(P<0.05或P<0.01)。结论:FFJZ可降低实验动物血液中脂质含量,减轻动脉血管内皮的损伤,抑制血管的炎性反应,可能是其抗动脉粥样硬化的机制之一。更多还原

【Abstract】 Objective: To study the influences of Fufang Jiangzhi capsule(FFJZ) on inflammatory factor in blood of rats with experimental atherosclerosis. Method: Twenty-five male SD rats and 25 female SD rats were given high diet(4% cholesterol,0. 5% sodium tauroglycocholate,0. 2% propylthiouracil,10% lard,85. 3% ordinary diet),then the rat AS model was established by injuring right common carotid artery and intramuscular injection of Vitamin D 3. The rats were randomly divided into five groups as follows: model group(saline),positive group(lovastatin 7. 48 mg·kg- 1),FFJZ high dose group(53. 34 g·kg- 1),FFJZ medium dose group(26. 67 g· kg- 1),low dose group(13. 33 g·kg- 1),respectively,while control group(10 rats) was given ordinary diet. The cupsules were given by oral administration once a day,after that,specimens of blood and right common carotid artery were collected,indications including blood-lipid,Interleukin-6(IL-6),Interleukin-10(IL-10) and tumor necrosis factor-a(TNF-α) were examined along with histopathology studies and observation of the change of artery walls with microscopy. Result: After the intervention treatment of the capsule,the concentrations of triglyceride(TG),total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),IL-6 were decreased while the concentrations of high density lipoprotein cholesterol(HDL-C),IL-10 and TNF-α were increased. The formation of arterial wall plaque was reduced; the deposits of foam cells and lipid were shorten. Conclusion: FFJZ could dramatically decrease the concentrations of lipid in serum and reduce risk of the formation of AS plaque. The mechanism could be associated with the inflammation inhibition,protecting the function of vascular endothelial cells and suppressing the formation of AS plaque.更多还原