【摘要】 目的:建立HPLC测定益心通脉合剂中丹参素钠、毛蕊异黄酮葡萄糖苷含量的方法。方法:丹参素钠的HPLC色谱条件为Welchrom-C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.5%乙酸(12∶88),流速1.0 mL·min-1,柱温30℃,进样量10μL,检测波长280 nm;毛蕊异黄酮葡萄糖苷的色谱条件为流动相乙腈(A)-0.2%甲酸溶液(B)梯度洗脱(0~13 min,13%~15%A;13~23 min,15%~20%A;23~30 min,20%~40%A;30~40 min,40%A),检测波长260 nm,其他条件同丹参素钠。结果:丹参素钠、毛蕊异黄酮葡萄糖苷的线性范围分别为0.213 44~5.336,0.078 72~2.361 6μg,平均加样回收率分别为100.17%(RSD 2.02%),101.79%(RSD 1.40%)。结论:建立的HPLC检测快速、定量准确、重复性较好,可用于益心通脉合剂的质量控制。更多还原

【Abstract】 Objective: To establish an HPLC method for determining sodium danshensu and calycosin-7-glucoside in Yixin Tongmai mixtures. Method: HPLC conditions of sodium danshensu were as follows: WelchromC18column(4. 6 mm × 250 mm,5 μm),mobile phase of methanol-0. 5% acetic acid(12∶ 88),flow rate 1. 0mL·min- 1,column temperature 30 ℃,injection volume 10 μL,detection wavelength 280 nm. But conditions of calycosin-7-glucoside were: mobile phase of acetonitrile(A)-0. 2% formic acid solution(B) with gradient elution program(0-13 min,13%-15% A; 13-23 min,15%-20% A; 23-30 min,20%-40% A; 30-40 min,40% A),detection wavelength at 260 nm; Other conditions were the same as sodium danshensu. Result: Linear ranges of sodium danshensu and calycosin-7-glucoside were 0. 213 44-5. 336 and 0. 078 72-2. 361 6 μg,respectively; Average recoveries of them were 100. 17%(RSD 2. 02%) and 101. 79%(RSD 1. 40%),respectively. Conclusion: This established HPLC method was rapid,accurate and reproducible,it could be used for quality control of Yixin Tongmai mixtures.更多还原