【摘要】 目的:优选茵陈四苓颗粒的乙醇提取工艺。方法:以栀子苷提取量为指标,通过单因素试验比较乙醇回流法、乙醇超声法和乙醇渗漉法,确定茵陈四苓颗粒中部分药材的提取方法;通过正交试验考察乙醇体积分数、浸泡时间、加醇量、渗漉流速对提取工艺的影响。采用HPLC测定栀子苷含量,色谱条件为UltimateTMC18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水(15∶85),流速1 mL·min-1,柱温40℃,检测波长238 nm,进样量10μL。结果:采用乙醇渗漉法提取,最佳提取工艺条件为加10倍量70%乙醇浸泡12 h,渗漉流速5 mL·min-1,栀子苷提取量达42.524 4 mg·g-1。结论:该工艺客观可行、稳定合理,可为茵陈四苓颗粒的工业生产提供理论依据。更多还原

【Abstract】 Objective: To optimize ethanol extraction technology of Yinchen Siling granules. Method:With yield of geniposide as index,single factor test was adopted to screen ethanol extraction method,such as refluxing method, ultrasonic method and percolation method; Effects of ethanol concentration, soaking time,ethanol amount and percolation velocity on extraction technology was investigated by orthogonal design. The content of geniposide was determined by HPLC,chromatographic conditions were as follows: UltimateTMC18column(4. 6mm × 250 mm,5 μm), mobile phase of acetonitrile-water(15 ∶ 85), flow rate 1 mL ·min- 1,column temperature 40 ℃,detection wavelength 238 nm,injection volume 10 μL. Result: Ethanol percolation method was adopted,the best extraction conditions were as follows: soaked 12 h by adding 10 times the amount of 70%ethanol with percolation velocity of 5 mL·min- 1; Under these conditions,yield of geniposide was up to 42. 524 4mg·g- 1. Conclusion: This optimized extraction process was stable,reasonable and practical,it could provide a theoretical basis for industrial production of Yinchen Siling granules.更多还原