Objective: To investigate relativity between the content of rutin and traditional specification grade of Farfarae Flos.Method: The content of rutin from Farfarae Flos was performed by HPLC on Dikma Diamonsil C18column(4.6 mm × 250 mm,5 μm) with acetonitrile-1% acetic acid(20∶80) as mobile phase and detection wavelength at 254 nm.HPLC fingerprint and similarity analysis of different specifications of Farfarae Flos was performed on Dikma Diamonsil C18column(4.6 mm × 250 mm,5 μm) with acetonitrile-0.1% H3PO4gr...