【摘要】 目的:研究滋阴方二至丸对二甲基苯蒽(DMBA)诱发性乳腺癌大鼠肿瘤组织中血管内皮生长因子(VEGF)、基质金属蛋白酶-9(MMP-9)表达的影响。方法:60只雌性SD大鼠随机分为空白对照组、DMBA模型组、二至丸高、中、低(6.4,3.2,1.6 g.kg-1)剂量组和三苯氧胺(TAM)组。除空白对照组外,每只大鼠均以致癌剂DMBA诱导(剂量100 mg.kg-1,2次,间隔1周)乳腺癌。诱导第2天开始给予药物干预,每周触诊所有大鼠乳腺肿瘤发生情况并记录体重变化;15周后处死动物,统计各组大鼠乳腺肿瘤的发病率,并采用SP免疫组化法检测各组动物肿瘤组织中VEGF,MMP-9的表达情况。结果:与DMBA模型组比较,二至丸中剂量组和TAM组大鼠乳腺肿瘤平均潜伏期延长(P<0.05),平均肿瘤直径、平均肿瘤体积、平均瘤重显著降低(P<0.05);二至丸中、高剂量组VEGF和MMP-9强阳性率显著性降低(P<0.05),且VEGF与MMP-9的表达呈正相关。结论:滋阴方药二至丸可能通过干预乳腺癌组织中VEGF和MMP-9的表达,抑制乳腺癌生长恶化。更多还原

【Abstract】 Objective: To explore the effect of Erzhi pills on expressions of vascular endothelial growth factor(VEGF) and matrix metalloproteinase-9(MMP-9) in brest cancer induced by 7,12-dimethylbenz(a) anthrancene(DMBA) in rats.Method: Sixty female SD rats were randomly divided into 6 groups: the blank control group,the DMBA model group,the high,middle,low dose(6.4,3.2,1.6 g.kg-1,respectively) Erzhi Pills group and tamoxifen(TAM) group.All model rats were induced by DMBA(100 mg.kg-1,twice,every two weeks),palpated weekly to monitor the progress of the tumor and body weight were also recorded weekly.At the end of trial,the incidence of breast tumors in each group was calculated.And the expressions of VEGF and MMP9 in breast cancer tissues were detected using immunohistochemical SP method.Result: Comparing with the DMBA model group,the breast cancer incidence,number and volume of tumor in the middle dose of Erzhi pills group and TAM group decreased significantly(P < 0.05).The strong-positive rates of VEGF and MMP-9 were significantly higher in the group treated with middle and high dose of Erzhi pills than DMBA model group(P < 0.05),and VEGF expression was positively correlated with MMP-9 expression in breast cancer.Conclusion: Erzhi pills might inhibit the progress and deterioration of breast cancer through regulating the expressions of VEGF and MMP-9.更多还原