【摘要】 目的:探讨雷公藤甲素对大鼠C6胶质瘤细胞的增殖诱导作用及其相关机制。方法:将大鼠C6胶质瘤细胞培养于含10%(FBS),100 mg.L-1青霉素链霉素的DMEM培养液中,置37℃5%CO2培养箱中培养,采用四甲基偶氮唑盐比色(MTT)法检测雷公藤甲素对大鼠C6胶质瘤细胞的抑制作用,流式细胞仪(FCM)检测C6胶质瘤细胞凋亡情况,用逆转录-聚合酶链反应(Real Time-Polymerase Chain Reaction,RT-PCR),免疫印迹法(Western blotting,WB)检测细胞淋巴瘤/白血病-2(Bcl-2),Bcl-2相关X蛋白(Bax)mRNA及蛋白的表达变化情况。结果:1.2 mg.L-1浓度的雷公藤甲素溶液对C6胶质瘤细胞增殖有抑制作用,且呈明显的剂量依赖性;雷公藤甲素作用C6胶质瘤细胞24 h后,1.2 mg.L-1浓度对其凋亡诱导作用最为明显;雷公藤甲素可明显下调细胞中Bcl-2 mRNA及蛋白质表达,且该作用呈剂量-时间依赖性,而Bax mRNA及蛋白质表达呈上调趋势。结论:雷公藤甲素对C6胶质瘤细胞具有较好的生长抑制和凋亡诱导作用,而其作用机制可能与Bcl-2,Bax mRNA及蛋白质表达有关。更多还原

【Abstract】 Objective: To investigate the effect of triptolide(TPL) on proliferation and apoptosis of C6 cells.Method: C6 cells was cultured with different concentrations of TPL for different hours.MTT assay was employed to evaluate the survival of TPL-treated C6 cells.The induction of apoptosis was detected by flow cytometry analysis(FCM).The expressions of B cell lymphoma / lewkmia-2(Bcl-2) and Bcl-2 assaciated x protein(Bax) mRNA were detected by Real Time-Polymerase Chain Reaction(RT-PCR),while the protein by Western blotting(WB) methods.Result: Our results showed that TPL induced apoptotic cell death in a dose-and timedependent manner,which was accompanied by the regulation of Bcl-2 and Bax expressions.Conclusion: The results indicated that TPL induce the apoptosis and inhibit the proliferation of C6 glioma cellsby regulating the expressions of Bcl-2 and Bax.更多还原