【摘要】 目的:观察益心脑滴丸对Wistar大鼠心肌缺血再灌注损伤的保护作用。方法:将健康成年大鼠随机分为6组:假手术(sham)组、缺血再灌注损伤(I/R)组、地奥心血康(DAXXK,0.07 g.kg-1)组、益心脑滴丸高(YXNH,3 g.kg-1)、中(YXNM,1.5 g.kg-1)、低剂量(YXNL,0.75 g.kg-1)组,连续预防口服给药7 d。采用结扎左冠状动脉前降支30 min、再灌注60 min的方法建立大鼠心肌缺血再灌注损伤模型。动态观察血流动力学各检测指标,心电图(ECG)变化,同时监测室性心律失常的变化。再灌注结束后,测定血清中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,测量心肌梗死面积。结果:与sham组比较,I/R组心率(HR)、左室收缩压(LVSP)、左室压最大上升和下降速率(±dp/dtmax)明显降低,左室舒张末期压(LVEDP)显著升高(P<0.01);SOD活性明显降低,MDA含量明显升高(P<0.01),且梗死区/缺血危险区(An/AAR)显著增大(P<0.05)。与I/R组相比,YXNH能明显升高HR,LVSP,±dp/dtmax,降低LVEDP,(P<0.01或P<0.05),降低室性心律失常的持续时间(P<0.01),增高SOD活性,降低MDA含量(P<0.01),明显减小An/AAR(P<0.01),其作用强度与DAXXK相似;YXNM和YXNL对I/R有保护作用,但没有DAXXK明显。结论:益心脑滴丸对I/R具有保护作用,其机制可能与改善心肌功能和抗氧自由基损伤有关。更多还原

【Abstract】 Objective:To observe the protective effect of Yixinnao dripping pills(YXN)on myocardial ischemia reperfusion injury(I/R)in Wistar rats.Method:The rats were randomly divided into six groups:sham-operated(sham)group,I/R group(I/R),Diao Xinxuekang(DAXXK,0.07 g·kg-1) treated group,high,medium and low dose groups pretreated by YXN(3,1.5,0.75 g·kg-1),each group orally administered 7 days.Then,the left anterior descending coronary artery was ligated for 30 min,followed by 60 min of reperfusion to establish the rat myocardial ischemia-reperfusion injury model.Hemodynamic and electrocardiogram(ECG) were observed continuously,and ventricular arrhythmias were measured.At the end of reperfusion,the serum malondialdehyde(MDA)concentration and superoxide dismutase(SOD)activity were measured.The myocardial infarct size was measured after reperfusion.Result:Heart rate(HR),left ventricular systolic pressure(LVSP),the maximum rise rate of left ventricular pressure(+dp/dtmax) and the maximum descending of left ventricular pressure(-dp/dtmax) and activity of SOD decreased obviously in I/R group compared with those in sham group;MDA content increased obviously(P<0.01)and the area of necrosis(An)/area at risk(AAR) was significantly elevated(P<0.05)in the I/R group.Compared with I/R,YXNH could significantly improve the HR,LVSP,±dp/dtmax and reduce the LVEDP(P<0.01 or P<0.05),decrease the lasting time of ventricular arrhythmias(P<0.01);the activity of SOD was obviously increased,and MDA content was decreased(P<0.01);the An/AAR was smaller(P<0.01).The effect was similar to that of DAXXK.YXNM and TXNL also had protective effect on I/R in rats,but their effect was not as good as DAXXK.Conclusion:YXN has protective action on I/R in rats,which may be related to promoting the functions of myocardium and enhancing the antioxidant ability.更多还原