【摘要】 目的:探索三七总皂苷注射液的超滤工艺。方法:采用HPLC测定人参皂苷Rg1,Rb1及三七皂苷R1的含量,通过单因素试验考察不同相对分子量超滤膜、药液质量浓度、温度、洗液体积对三七总皂苷注射液超滤工艺的影响。结果:确定的超滤工艺为三七总皂苷水溶液质量浓度100 g.L-1,选用10 kDa超滤膜,超滤温度70℃,洗液体积1 200 mL,人参皂苷Rg1,Rb1及三七皂苷R1各组份透过率均>90%。结论:优选的超滤工艺用于三七总皂苷注射液的纯化是可行的。更多还原

【Abstract】 Objective:To explore ultrafiltration technology of total saponin from Panax notoginseng injection.Method:Contents of ginsenoside Rg1,Rb1 and notoginsenoside R1 were determined by HPLC,effect of different factors on ultrafiltration technology were investigated by single factor test,including different molecular weight ultrafiltration membrane,the concentration and temperature of solution,lotion volume.Relust:10 kDa ultrafiltration membrane was chosen,determined ultrafiltration technology was:the mass concentration of water solution of 100 g · L-1,ultrafiltration temperature 70 ℃,lotion volume 1 200 mL,transmission of ginsenoside Rg1,Rb1 and notoginsenoside R1 were more than 90%.Conclusion:This optimized ultrafjltration technology was feasible for purification of total saponin of P.notoginseng.更多还原