【摘要】 relish是Rel/NF-κB家族的重要成员之一。本研究在构建的斑节对虾(Penaeus monodon)肝胰腺转录组文库中克隆得到PmRelish基因,其全长5 112 bp,包括3 561 bp的ORF,推测编码1个含1 186个氨基酸的蛋白。PmRelish的蛋白具有Rel/NF-κB蛋白家族的典型结构特征,包括N端的RHD结构域,核定位信号,C端的6个锚蛋白重复序列以及死亡结构域,表明该蛋白是NF-κB1亚型。该基因在所检测组织中呈组成型表达,并在血细胞表达量最高。使用定量PCR检测PmRelish基因在不同免疫刺激条件下在肝胰腺中的mRNA表达情况,结果显示,肝胰腺PmRelish基因是诱导型表达,并被创伤弧菌、金黄色葡萄球菌和白斑综合征病毒(WSSV)调控表达,其中被创伤弧菌上调表达最显著。进一步证明了病毒核酸类似物Poly(I:C)、R484可激活PmRelish的表达。上述研究结果说明Relish是斑节对虾的重要免疫因子,可能通过直接或间接作用参与对细菌和病毒的免疫反应。更多还原

【Abstract】 Relish is an important member of the Rel/NF-kB family.We cloned a Relish homologue,Pmrelish,from the hepatopancreas transcriptome library of Penaeus monodon.The full-length cDNA of Pmrelish was 5 112 bp with an open reading frame of 3 561 bp that encodes 1 186 amino acids.PmRelish protein exhibits the typical architecture of Rel/NF-κB family members,including a conserved N-terminal Rel homology domain(RHD),a nucleus localization signal,six C-terminal ankyrin repeats,and a death domain,suggesting that it belongs to the class I NF-κB.PmRelish mRNA was constitutively expressed in all detected tissues,with the highest expression in the hemocytes.Using real time PCR,we analyzed the mRNA expression profile of PmRelish following challenge with a variety of stimuli.The results show that PmRelish was induced in the hepatopancreas.PmRelsih was regulated by Vibrio vulnificus,Staphylococcus aureus,and WSSV,and significantly up-regulated by Vibrio vulnificus.In addition,we confirmed that viral nucleic acid analogue poly(I:C) and R484 activate the expression of PmRelish.In conclusion,our results suggest the Relish gene plays an important role in the immunity of tiger shrimp,and is directly or indirectly involved in the immune response against bacteria and viruses.更多还原