【摘要】 目的通过检测Tim-3mRNA的表达水平及IFN-γ、IL-4的含量,初步探讨肺炎支原体感染鼠Th1/Th2细胞的动态变化及Tim-3的免疫调控作用。方法通过滴鼻感染建立肺炎支原体肺炎小鼠模型,观察小鼠的一般状况,分别于感染后3d、5d、7d、14d、21d取材,应用RT-PCR方法检测肺组织和脾脏Tim-3mRNA的表达;采用ELISA法检测肺泡灌洗液中细胞因子IFN-γ、IL-4的含量。结果实验组IFN-γ于3d出现升高(t=2.309,P<0.05),5dIFN-γ开始下降,而IL-4于3d开始升高并一直持续到7d(t=6.436,P<0.05),之后逐渐恢复正常,肺组织和脾脏Tim-3mRNA的表达均于5d达到峰值(t=4.727,P<0.05;t=7.962,P<0.05),且肺组织和脾脏Tim-3mRNA表达量与IL-4呈正相关(r=0.561,P<0.05;r=0.462,P<0.05),从5d开始与IFN-γ呈负相关(r=-0.379,P<0.05;r=-0.431,P<0.05)。结论小鼠感染肺炎支原体后Th1/Th2失衡,以Th2介导的免疫反应为主;Tim-3可能参与了感染后对Th1细胞的负调控。更多还原

【Abstract】 In this article,we study the expression of Tim-3 mRNA and the Th1/Th2 status in mice with Mycoplasma pneumoniae(Mp)pneumonia.The Mycoplasma pneumoniae(Mp)infection model was built by inhalation in BALB/c mice,and the general condition was observed.Tim-3 mRNA levels in lung tissue and spleen were determined by RT-PCR.The levels of IL-4 and INF-γin BALF were measured by ELISA.The results showed that compared with control group,the IFN-γlevel in the experimental group increased significantly at day 3p.i.(P<0.05)and gradually declined at day 5p.i.(P>0.05),but the IL-4 level in the experimental group increased significantly at day 3p.i.and continued to the day 7p.i.(P<0.05).The expression of Tim-3 mRNA in lung and spleen were both the highest at day 5p.i.(t=4.727,P<0.05;t=7.962,P<0.05),and were positively correlated with IL-4 level(r=0.561,P<0.05;r=0.462,P<0.05),but negatively correlated with INF-γlevel from day 5p.i.(r=-0.379,P<0.05;r=-0.431,P<0.05).In conclusion,after the infection of Mp,there was an imbalance of Th1/Th2 cells and Th2 cells dominated.Tim-3 might probably produce a negative regulatory against Th1 cells after Mycoplasma pneumoniae infection,but its mechanism needs further study.更多还原