【摘要】 目的建立适合慢性胃炎相关TTV病毒增殖的细胞培养体系,观察病毒的超微形态。方法制备人胃腺癌SGC-7901和人胃粘膜细胞GES-1传代细胞,对TTV病毒进行传代,培养至有细胞病变出现,并对接毒细胞TCID50进行动态检测;收集病毒感染细胞,制备电镜样品,使用透射电子显微镜进行超微形态观察。结果盲传3代后,接毒72hSGC-7901细胞即可产生明显的细胞病变;TTV病毒在SGC-7901上的TCID50随传代次数的增加而增高,由F1代101.2快速增至F4代106.0,之后稳定在106.0~106.8之间;盲传8代后,TTV病毒在GES-1细胞上细胞病变仍不明显;透射电镜观察结果显示,TTV病毒在细胞核和胞质内均有分布,病毒呈球形,直径20nm左右,无囊膜。结论 SGC-7901细胞可以作为TTV病毒体外增殖的培养体系,已经获得适合SGC-7901细胞增殖的TTV病毒。更多还原

【Abstract】 The aim is to establish a suitable proliferative cell culture system for TTV(Transfusion transmitted virus),and observe the ultramicro-morphology of this virus.The gastric adenocarcinoma cell SGC-7901and gastric mucosal cell GES1were prepared,and the TTV in passage SGC-7901and GES-1were subcultured until the cytopathic effect(CPE)appeared.Dynamic detection of TCID50 and observation on transmission electron microscopy(TEM)of infected cells were carried out.After 3generation blind passage in SGC-7901,TTV could produce obvious CPE 72hours p.i.The TCID50 titer of TTV was getting higher from F1 101.2 to F4 106.0,and then stabilized between 106.0and 106.8.But in GES-1,after 8generation blind passage the CPE was still atypism.The observation of TEM showed that TTV were distributed in the cytoplasm and nucleus of the infected cells.The virions were globular and unenveloped,and the average diameter was 20nm.SGC-7901cells can be used as a proliferation system for TTV,and the SGC-7901cell adapted TTV virus have been obtained invitro.更多还原