【摘要】 目的:观察肥胖乳腺癌患者乳腺癌组织中磷酯酰肌醇3激酶(PI3K)/Akt信号通路的活性。方法:选取45例肥胖乳腺癌患者(肥胖组)与37例正常体质量乳腺癌患者(对照组)的乳腺癌组织标本,分别用RT-PCR法检测两组乳腺癌组织中PI3K mRNA的表达,及免疫组化法检测PI3K和磷酸化Akt(p-Akt)蛋白的表达。结果:RT-PCR结果显示,肥胖组乳腺癌组织中PI3K mRNA表达较对照组明显增高(P<0.05);免疫组化结果显示,肥胖组PI3K和p-Akt蛋白表达均明显升高(均P<0.05),且PI3K与p-Akt蛋白表达水平在两种组织中均呈正相关(r=0.76,r=0.81,均P<0.05)。结论:肥胖乳腺癌患者癌组织中PI3K/Akt信号通路活性明显高于非肥胖患者,故推测这可能是导致肥胖者乳腺癌风险增高的原因之一。更多还原

【Abstract】 Objective: To observe the activity of the signaling pathway of phosphatidylinositol 3-kinase(PI3K)/Akt in the tumor tissues in obese breast cancer patients. Methods: Breast cancer specimens from 45 obese breast cancer patients(obesity group) and 37 normal-weight breast cancer patients(control group) were collected, and then, the PI3K mRNA expression was determined by RT-PCR, and the protein expressions of PI3K and phosphorylated Akt(p-Akt) were determined byimmunohistochemical staining in the two groups of breast cancer tissues, respectively. Results: The results of RT-PCR showed that the PI3K mRNA expression was signiicantly elevated in breast cancer tissues of obesity group compared with control group(P<0.05). The results of immunohistochemical staining revealed that both PI3K and p-Akt protein expressions in breast tissues of obesity group were signiicantly higher than those in breast tissues of control group(both P<0.05) and, moreover there was a positive correlation between PI3K and p-Akt protein expression in either group of tissues(r=0.76 and r=0.81, both P<0.05). Conclusion: The activity of PI3K/Akt signaling pathway in cancer tissues of obese breast cancer patients is increased compared with non-obese breast cancer patients, thus, it is speculated to be one of the factors for the increased risk of breast cancer in obese women.更多还原