【摘要】 目的探讨尾型同源盒转录因子-2(CDX-2)和抑癌基因KAI-1在结肠癌中的表达并分析其临床意义。方法应用免疫组织化学SP法检测50例结肠癌组织及相应癌旁组织和25例正常结肠黏膜组织中CDX-2和KAI-1蛋白的表达,分析其表达与结肠癌患者临床病理特征的关系以及二者表达的相关性。结果①在结肠癌组织及相应癌旁组织(距癌组织≤2 cm)和正常结肠黏膜组织中CDX-2蛋白表达阳性率分别为34%(17/50)、54%(27/50)及88%(22/25),KAI-1蛋白表达阳性率分别为30%(15/50)、58%(29/50)及92%(23/25),CDX-2和KAI-1在结肠癌组织中的蛋白表达阳性率均分别明显低于相应癌旁组织(P<0.05)及正常结肠黏膜组织(P<0.05),其在癌旁组织中的表达阳性率也均明显低于正常结肠黏膜组织(P<0.05)。②CDX-2和KAI-1蛋白表达阳性率与结肠癌淋巴结转移、浸润深度及分化程度均有关(P<0.05),即在有淋巴结转移、浸润深度浸及浆膜及分化程度较低者中CDX-2和KAI-1蛋白表达阳性率均明显低于其在无淋巴结转移、浸润深度未及浆膜及分化程度较高者(P<0.05),二者均与患者的发病年龄和性别无关(P>0.05)。③Spearman等级相关分析表明,CDX-2和KAI-1蛋白阳性表达呈正相关(r s=0.544,P<0.01)。结论 CDX-2和KAI-1的表达可能与结肠癌的发生、发展、浸润、转移及预后相关,联合评价其功能可能对结肠癌治疗具有一定指导意义。更多还原

【Abstract】 Objective To explore the expressions of caudal-related homeodomain transcription factor-2(CDX-2)and tumor suppressor gene KAI-1 in colon carcinoma tissues and to analyze their clinical significances. Methods Immunohistochemical SP method was used to detect the expressions of CDX-2 and KAI-1 in 50 cases of colon carcinoma tissues and corresponding adjacent tissues(from cancer tissue ≤2 cm)and 25 cases of normal colon mucosa tissues. The relationships of the expressions of CDX-2 and KAI-1 to the clinicopathologic features were analyzed. Results ① The positive rates of CDX-2 expression and KAI-1 expression in the colon carcinoma tissues were significantly lower than those in the normal colon mucosa tissues〔CDX-2:34%(17/50)versus 88%(22/25),P< 0.05;KAI-1:30%(15/50)versus 92%(23/25),P<0.05〕and adjacent tissues of colon carcinoma〔CDX-2:34%(17/50)versus 54%(27/50),P<0.05;KAI-1:30%(15/50)versus 58%(29/50),P<0.05〕,which in the adjacent tissues of colon carcinoma were significantly lower than those in the normal colon mucosa tissues(P<0.05). ② The positive expressions of CDX-2 and KAI-1 were related to lymph node metastasis,depth of invasion,and degree of tumor differentiation(P<0.05). ③ Spearman rank correlation analysis showed that the CDX-2 expression was positively correlated with KAI-1 expression(rs=0.544,P<0.01).Conclusions CDX-2 and KAI-1 may be closely related to the development,invasion,metastasis,and prognosis of colon carcinoma,the combination of CDX-2 and KAI-1 in evaluation of their function has a certain guiding significance in the treatment for colon carcinoma.更多还原