【摘要】 目的:分析可溶性Fas(soluble Fas,sFas)表达水平与人黑色素细胞瘤恶性程度的关系,探讨Fas、Fas配体与可溶性Fas在调控细胞凋亡信号转导途径中的作用。方法:对35位确诊黑色素细胞癌患者的皮肤组织标本和病例进行回顾性分析和归类,用免疫荧光的方法检测组织中Fas/FasL的表达水平。通过酶联免疫吸附反应(ELISA)方法对人血清中sFas的浓度进行分析。结果:35例黑色素细胞癌组织中,病理分型浅表型2例、结节型9例、肢端型18例、雀斑痣型6例。Fas在正常皮肤组织中高表达,恶性黑色素癌组织中低表达;而相反FasL在恶性黑色素癌组织中高表达,在正常皮肤组织中表达非常低。可溶性Fas在不同组织中的表达强度差异显著,sFas在恶性黑色素细胞癌患者、良性黑色素瘤和正常人血清中的浓度分别为(36.32±11.57)ng/ml、(7.33±3.78)ng/ml和(4.18±1.88)ng/ml。结论:可溶性Fas在恶性黑色素癌患者血清中含量较高,与黑色素细胞癌恶性程度呈正相关,sFas对细胞凋亡的竞争性抑制可能与黑色素细胞癌的进展密切相关。更多还原

【Abstract】 Objective:To investigate the apoptosis related mechanisms of Fas/Fas ligand and soluble Fas in malignant melanomaby by analyze soluble Fas(sFas) expression in primary human malignant melanoma.Methods:Analyze the pathological and disease staging records by reviewing 35 cases of human malignant melanoma,determine Fas/FasL expression by immunoflourescence.Analyze soluble Fas expression levels in serum samples of patients by ELISA.Results:In 35 cases of malignant melanoma,there were 2 cases of superficial spreading melanoma,9 cases of nodular melanoma,18 cases of acral letiginous melanoma and 6 cases of lentigo maligna melanoma.Fas expression was high in normal skin tissues and low in malignant melanomas,when FasL expression was the opposite.The soluble Fas levels showed significant differences in malignant melanoma,compare to melanocytic nevus and normal people were(36.32±11.57) ng/ml,(7.33±3.78) ng/ml and(4.18±1.88) ng/ml respectively.Conclusion:Soluble Fas was highly expressed in malignant melanoma,which is correlated to the malignancy.Soluble Fas may competitively play important roles in apoptosis mechanisms of malignant melanoma.更多还原