【摘要】 目的探讨CD4+T细胞Bcl-6与Blimp-1在慢性乙型肝炎发病机制中的作用。方法密度梯度离心法分离外周血单个核细胞(PBMC),免疫磁珠法从中分选出CD4+T细胞,半定量PCR法检测CD4+T细胞中Bcl-6与Blimp-1表达水平。结果与健康对照比较,慢性乙型肝炎患者中Bcl-6 mRNA表达升高,Blimp-1 mRNA表达降低,差异均有统计学意义(P<0.05)。结论慢性乙型肝炎患者CD4+T细胞Bcl-6与Blimp-1转录因子的表达存在明显异常,可能与慢性乙型肝炎异常免疫应答有关。更多还原

【Abstract】 Objective To investigate the effect of Bcl-6(B cell lymphoma/leukemia 6) and Blimp-1(Blymphocyte induced maturation protein-1)in the pathogenesis of chronic hepatitis B. Methods Peripheral anticoagulant Blood were collected and CD4+T cells from PBMC(Peripheral Blood Mononuclear Cell) were sorted by the methods of immunomagnetic bead.The expression of Blimp-1 mRNA and Bcl-6 mRNA levels were detected by reverse transcription-polymerase chain reaction(RT-PCR). Results Compared with the healthy controls, Bcl-6mRNA expression was higher in patients with chronic hepatitis B, while the expression of Blimp-1 mRNA was weaker. Conclusions The expression of Bcl-6 mRNA and Blimp-1 mRNA levels were significant differences in chronic HBV patients. This may be related to the abnormal immune responses of chronic hepatitis B.更多还原