【摘要】 目的观察大鼠心肌梗死(MI)后经静脉移植的人羊膜间充质干细胞(hAD-MSCs)在体内的定植、分化及对心功能的影响。方法SD大鼠随机分为hAD-MSCs移植组,模型组和假手术组各12只。分离hAD-MSCs用流式细胞仪和免疫组化染色鉴定其表型特征。制模后1 w经舌下静脉移植Brdu标记的hAD-MSCs,于移植后6 w将大鼠心脏取出并切片行组织病理学、免疫组织化学、双重免疫荧光染色等检测,以观察植入的细胞在MI区的定植、分化,在肝、脾中的分布情况。结果分离的hAD-MSCs具有典型的间充质干细胞表型特征,高表达CD44和波形蛋白;静脉移植后6 w,心脏MI区、肺脏和脾脏中均可见Brdu阳性细胞,但在肝脏中未见阳性细胞,移植的hAD-MSCs表达心肌特异蛋白连接蛋白-43和α-辅肌动蛋白。移植后6 w,移植组大鼠心功能没有进一步恶化。结论 hAD-MSCs经静脉移植能够定植于大鼠MI区域,可分化为心肌细胞,并改善心功能。更多还原

【Abstract】 Objective To observe the distribution and differentiation of hAD-MSCs injected intravenously and the feasibility of it used to repair the infracted heart.Methods SD rats were randomly assigned into hAD-MSCs transplanted,model and sham operation groups(12 rats each group).The hAD-MSCs were isolated from human amnion and then its phenotype was identified by flow cytometry and immunohistochemical staining.hAD-MSCs were injected into the subglossal vein of the SD rats 7 days after myocardial infarction(MI) was created and the organs such as the heart,liver and spleen were harvested at 6 weeks after transplantation.In order to assess the survival and differentiation of hAD-MSCs posttransplantation in the MI zone,as well as the influence on cardiac function.Results The isolated hAD-MSCs had the typical features of mesenchymal stem cells(MSCs),high expression of CD44,CD73,CD90,CD105 and vimentin.After injection through the subglossal vein,the hAD-MSCs passed through the capillary of the lung,and then distributed into the heart,lung and spleen,but seldom in the liver.At 6 weeks after hAD-MSCs transplantation,cell engraftments expressed cardiac-specific protein connexin-43 and α-actinin.The left ventricle function did not exacerbate after injection of hAD-MSCs compared with that in control group.Conclusions hAD-MSCs injected intravenously can distribute into myocardium,lung and spleen.The migration of injected cells delays the left ventricle remodeling after MI.更多还原