【摘要】 目的:探讨萝卜硫素(SF)对白血病细胞化疗药物敏感性的作用。方法:琼脂半固体集落培养法和MTT法检测不同浓度的萝卜硫素对HL-60细胞增殖的影响及协同化疗药对细胞生长的抑制作用;PHA-LCM液相-半固体二步培养SF协同化疗药抑制人原代白血病细胞生长。结果:琼脂半固体集落培养法和MTT法证实在一定浓度范围内SF对HL-60细胞增殖抑制(P<0.05,P<0.01),呈剂量依赖性,SF能够增强HL-60细胞对化疗药物高三尖杉酯碱(Hom)和阿糖胞苷(Ara-c)敏感性(P<0.05);PHA-LCM液相-半固体二步培养法证实SF在一定浓度范围内对人原代白血病细胞有抑制作用(P<0.05,P<0.01),呈剂量依赖性,并增加人原代白血病细胞对化疗药Hom和Ara-c敏感性。结论:萝卜硫素在一定浓度下有效抑制白血病细胞增殖,并能增强化疗药物的敏感性。更多还原

【Abstract】 Objective: To investigate the effect of sulforaphane(SF) on chemotherapy sensitivity of leukemia cells.Methods: Semi-solid agar colony culture assay and MMT method were used to observe the effect of different concentrations of SF on HL-60 cell proliferation and homoharringtonine+ ara-c chemotherapy on cell growth.PHALCM two-stage culture method was used to detect the effect of SF and homoharringtonine+ara-c chemotherapy on human primary leukemia cells.Results: With a certain range of concentrations,SF inhibited the proliferation of HL-60 cells and the growth of human primary leukemia cells in a dose-dependent manner(P<0.05,P<0.01).SF enhanced homoharringtonine+ara-c chemotherapy sensitivity of HL-60 cells(P<0.05) and human primary leukemia cells.Conclusion: SF can inhibit the proliferation of leukemia cells with a certain range of concentrations and enhance chemotherapy sensitivity of these cells.更多还原