【摘要】 目的:观察肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)对人脐静脉内皮细胞(ECV304)蛋白C受体(EPCR)mRNA表达的影响。方法:通过体外培养ECV304细胞,分别以含TNF-α和IL-1β的培养基孵育ECV304细胞,行剂量和时间依赖性实验。采用实时定量聚合酶链反应(quantitative real-time PCR)技术测定ECV304细胞EPCR mRNA的表达。结果:TNF-α和IL-1β均能显著下调ECV304 EPCR mRNA的表达,并呈剂量和时间依赖性(P<0.05)。结论:TNF-α和IL-1β可能通过显著下调ECV304上EPCR mRNA的表达而成为损伤内皮细胞功能的一个新作用机制。更多还原

【Abstract】 Objective:To investigate the effect of tumor necrosis factor alpha(TNF-α) and interleukin-1 beta(IL-1β) on the expression of endothelial protein C receptor(EPCR) in human umbilical vein endothelial cells(ECV304).Methods: Cultured ECV304 were used as target cells.Different concentrations of TNF-α or IL-1β were added to the culture medium to establish the injury model of ECV304.EPCR mRNA were measured by quantitative real-time PCR.Results: The expression of EPCR in ECV304 was down-regulated by TNF-α and IL-1β in dose and time dependent manner.Conclusion: Both TNF-α and IL-1β may damage the function of endothelial cells by down-regulating the expression of EPCR mRNA.更多还原